[BioC] HTqPCR - how to read data file with multiple samples
Adam Kiezun
akiezun at gmail.com
Thu Jan 7 23:07:13 CET 2010
I have a data file (created by SDS), with 6 samples, like this:
# Status Sample Detector Task Avg Ct
1 Analyzed 1 MammU6-4395470 Endogenous Control 22.733
2 Analyzed 2 MammU6-4395470 Endogenous Control 22.582
3 Analyzed 3 MammU6-4395470 Endogenous Control 22.656
4 Analyzed 4 MammU6-4395470 Endogenous Control 22.785
5 Analyzed 5 MammU6-4395470 Endogenous Control 22.684
6 Analyzed 7 MammU6-4395470 Endogenous Control 22.411
I tried running:
readCtData("Data.txt", SDS=TRUE, n.features=381, type=5, Ct=6,
header=TRUE, feature=4)
the HTqPCR package reads the data file as if there were only 1 sample:
An object of class "qPCRset"
Size: 381 features, 1 samples
Feature types: Endogenous Control, Target
Feature names: MammU6-4395470 MammU6-4395470 MammU6-4395470 ...
Feature classes:
Feature categories: OK
Sample names: Data NA NA ...
How do I read the data into HTqPCR so that it recognizes that I have 6
samples?
./adam
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