[BioC] HTqPCR - how to read data file with multiple samples

Adam Kiezun akiezun at gmail.com
Thu Jan 7 23:07:13 CET 2010


I have a data file (created by SDS), with 6 samples, like this:
#  	Status	Sample	Detector	Task	Avg Ct
1	Analyzed	1	MammU6-4395470	Endogenous Control	22.733	
2	Analyzed	2	MammU6-4395470	Endogenous Control	22.582	
3	Analyzed	3	MammU6-4395470	Endogenous Control	22.656	
4	Analyzed	4	MammU6-4395470	Endogenous Control	22.785	
5	Analyzed	5	MammU6-4395470	Endogenous Control	22.684	
6	Analyzed	7	MammU6-4395470	Endogenous Control	22.411	

I tried running:

  readCtData("Data.txt", SDS=TRUE, n.features=381, type=5, Ct=6,
header=TRUE, feature=4)

the HTqPCR package reads the data file as if there were only 1 sample:

  An object of class "qPCRset"
  Size:  381 features, 1 samples
  Feature types:		 Endogenous Control, Target
  Feature names:		 MammU6-4395470 MammU6-4395470 MammU6-4395470 ...
  Feature classes:	
  Feature categories:	 OK
  Sample names:		 Data NA NA ...

How do I read the data into HTqPCR so that it recognizes that I have 6
samples?

./adam



More information about the Bioconductor mailing list