[BioC] Problem reading files on FlowCore
Nishant Gopalakrishnan
ngopalak at fhcrc.org
Thu Feb 18 20:38:50 CET 2010
Hi Sancho,
Each line of code in a vignette starts with a ">" . Parts of a code
chunk that does not fit into a single line and get carried over to the
next line get a "+" at the beginning of the line.
Additionally, the phenoData argument is case sensitive. (You have used
phenodata)
It would be a good idea to get familiar with the syntax of R before
attempting to go through work flows.
Nishant
Sancho, Jose wrote:
> I am afraid I do not know. I am following the instructions as set on
> Flow cytometry data analysis using Bioconductor - A typical work flow.
>
> Nishant Gopalakrishnan
>
> July 23, 2009
>
>
>
> And I can't load the fcs files.
>
>
>
> -----Original Message-----
> From: Chao-Jen Wong [mailto:cwon2 at fhcrc.org]
> Sent: Wednesday, February 17, 2010 6:04 PM
> To: Sancho, Jose
> Cc: bioconductor at stat.math.ethz.ch
> Subject: Re: [BioC] Problem reading files on FlowCore
>
>
>
> Hi Jose,
>
>
>
> May I ask why there is a "+" sign before the transformation argument?
>
>
>
> -C.J.
>
>
>
> Sancho, Jose wrote:
>
>
>> I am new to FlowCore, and I am trying to get the demo files,as
>>
> described
>
>
>> on Flow Cytometry data analysis using Bioconductor- A typical work
>>
> flow,
>
>
>> and having difficulties.
>>
>
>
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>>
>>
>
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>> I type the instruction flowData <-read.flowSet(path = "C:\Documents
>>
> and
>
>
>> Settings\sanjo05\My Documents\BIOCONDUCTOR SOFTWARE", phenodata =
>>
>
>
>> "annotation.txt", + transformation = FALSE).
>>
>
>
>
>
>>
>>
>
>
>
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>> I get the following error message Error: unexpected '=' in "flowData
>>
>
>
>> <-read.flowSet(path = "C:\Documents and Settings\sanjo05\My
>>
>
>
>> Documents\BIOCONDUCTOR SOFTWARE", "annotation.txt", + transformation
>>
> ="
>
>
>
>
>>
>>
>
>
>
>
>> What am I doing wrong?
>>
>
>
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>>
>>
>
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>
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>> Thanks
>>
>
>
>
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>>
>>
>
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>> Jose Sancho
>>
>
>
>
>
>>
>>
>
>
>
>
>> Genzyme Corporation
>>
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>
>> Flow Cytometry Core Facility
>>
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>> [[alternative HTML version deleted]]
>>
>
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>>
>
>
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>>
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>>
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>
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>>
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