[BioC] Problems running oneChannelGUI

Mark Cowley m.cowley at garvan.org.au
Fri Dec 3 01:18:05 CET 2010


Hi Marcos,
i'm not familiar with oneChannelGUI, but I think the problem is that  
the full path to that file has a space in it. Can you move your file  
to a location with no spaces in the path & try again? ... or just  
rename Microarray DATA to Microarray_DATA

cheers,
Mark

On 03/12/2010, at 12:07 AM, Marcos Pinho wrote:

> Dear List,
>
> I am having some problems running oneChannelGUI to analyze some  
> HuGene 1.0
> ST arrays.
>
> I have installed all the necessary components according to the  
> installation
> instructions. The program opens fine, I am able to select my working
> diretory and my RNA target file. The problem occurs when I try to  
> normalize
> my data. I keep getting the following error message:
>
>
> Gene level probe sets summary started
>
> FATAL ERROR: .\TsvFile\TsvFile.cpp:2482: open: Could not open file:
> 'C:/Users/mbpinho/Documents/Microarray' to read.
>
> Gene level probe sets summary ended
> Warning messages:
> 1: In file(file, "rt") : only first element of 'description'  
> argument used
> 2: In file(file, "rt") :
>  cannot open file 'C:/Users/mbpinho/Documents/Microarray DATA/INCA
> Data/Uniscience/out6db54a37/': No such file or directory
>>
>
> This problem only occurs when I am trying to analize the new Hugene  
> 1.0 ST
> arrays, if instead I use other data with 3' IVT arrays, the software  
> runs
> fine. I have already installed the APT tools from Affymetrix as well  
> as the
> Tcl/Tk extensions.
>
> Any suggestions on how to fix my problem??
>
> Regards,
> -- 
> Marcos B. Pinho
> Programa de Engenharia Química - PEQ
> Laboratório de Engenharia de Cultivos Celulares- LECC
> Universidade Federal do Rio de Janeiro - UFRJ
> Instituto Nacional de Câncer - INCA
> Rio de Janeiro - Brasil
>
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>
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