[BioC] topGO and Arabidopsis data
Johannes Hanson
s.j.hanson at uu.nl
Thu Dec 2 17:44:32 CET 2010
Dear All,
I am analyzing affymetrix expression data using the topGO package.
I basically follow the script in the topGO vingette. It works fine for the CC and BP ontologies but for MF i get the following error:
> sampleGOdata <- new("topGOdata", description = "Simple session", ontology = "MF", allGenes = geneList, geneSel = topDiffGenes, nodeSize = 10, annot = annFUN.db,affyLib = affyLib)
Building most specific GOs ..... ( 1348 GO terms found. )
Build GO DAG topology ..........
There are no adj nodes for node: GO:0010241
Error in switch(type, isa = 0, partof = 1, -1) :
EXPR must be a length 1 vector
I guess that there is something wrong in the annotation packages but honestly, I might well have misunderstood the error message. Using the examples in the topGO vingette and human annotation no errors are given.
Thanks in advance,
Johannes
> sessionInfo()
R version 2.12.0 (2010-10-15)
Platform: x86_64-apple-darwin9.8.0/x86_64 (64-bit)
locale:
[1] en_GB.UTF-8/en_GB.UTF-8/C/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] topGO_2.2.0 SparseM_0.86 GO.db_2.4.5 graph_1.28.0 ath1121501.db_2.4.5 org.At.tair.db_2.4.6 RSQLite_0.9-3
[8] DBI_0.2-5 AnnotationDbi_1.12.0 ath1121501cdf_2.7.0 affy_1.28.0 Biobase_2.10.0
loaded via a namespace (and not attached):
[1] affyio_1.18.0 grid_2.12.0 lattice_0.19-13 preprocessCore_1.12.0 tools_2.12.0
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