[BioC] analysis of differential expression of small RNAs sequences
anders at embl.de
Sat Apr 17 10:11:02 CEST 2010
On Thu, 15 Apr 2010 13:50:19 +0100, Andreia Fonseca wrote:
> I have sequences of small RNAs which were produced using the GAII,
> I have my counts table and I need to test which sequences have different
> expression between treatments. I have started to use DEseq package. But
> would like to know if it correct to use the Deseq package which is fro
> RNAseq or if there is a package for small RNA sequences.
DESeq does not make any assumption about the type of data, except for that
they are counts. So, using it for small RNA-Seq is perfectly fine.
The step before, i.e., making the count table, is what depends on the
assay type. For small RNA, you may have more problems with ambiguous
matches, sequencing into adapters, etc., but it seems that you have
dealt with this.
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