[BioC] Different expression direction between limma microarray data analysis vs quantitative real time PCR result

[Naomi Altman]

False discoveries?

--Naomi

At 12:38 AM 9/21/2009, jixinwang at tamu.edu wrote:
>Dear all, I use limma package to do microarray 
>data analysis and most of real time PCR 
>validation results are consistent with 
>microarray data in terms of expression direction 
>and statistical significance. However in one set 
>of experiemnts, two DE genes are statistically 
>significant in both of microarray and qRT-PCR 
>result BUT had the different expression 
>direction. (e.g. They are  down regulated in 
>microarray result but real time PCR result 
>showed that they are up regulated)? I don’t 
>know why. Does this occur to anyone before? Many 
>thanks.  Best regards, Wang 
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111