[BioC] flowCore: inverse logicle transformation of flow cytometry data

Chao-Jen Wong cwon2 at fhcrc.org
Thu Sep 17 00:24:36 CEST 2009


Hi, Pyne

That is an interesting question. flowCore does not have an inverse
function for the logicle transformation. Since the logicle
transformation is an one-to-one and onto function, it is possible to
implement an inverse function. It is, however, not straightforward.  Do
you really really need such a function?

spyne at broadinstitute.org wrote:
>
> Hi,
>
> I applied logicle transformation (with default arguments)
> to my data points, then detected the subpopulations of
> interest in the transformed data, and now I want to
> revert the subpopulations back to the original scale of
> the untransformed state.
>
> In other words, if I want to apply the inverse of the logicle
> transformtion (applied with default arguments, which I do not
> know) to my data, is that possible?
>
> Thanks.
> -Pyne
>
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-- 
Chao-Jen Wong
Program in Computational Biology
Division of Public Health Sciences
Fred Hutchinson Cancer Research Center
1100 Fairview Avenue N., M2-B876
PO Box 19024
Seattle, WA 98109
206.667.4485
cwon2 at fhcrc.org



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