[BioC] Error in ebayes: No residual degrees of freedom in linear model fits

[Naomi Altman]

You need replication. --Naomi

At 04:25 PM 5/25/2009, Lucas Santana dos Santos wrote:
>Dear Group,
>
>I am trying to find differential expressed genes using Limma, however
>I got the following error when I try eBayes:
>
>Error in ebayes(fit = fit, proportion = proportion, stdev.coef.lim =
>stdev.coef.lim) :
>No residual degrees of freedom in linear model fits
>
>My dataset consists of 8 arrays from 8 different cell lines.  I have
>no replicates.
>I just want to see which genes are differentially expressed between
>these cell lines.
>
>I am wondering if I need more than one array per cell line in order to
>make this work.
>Maybe eBayes need replicates to generate variability. Does it make
>sense?
>
>A similar post already exists: 
>https://stat.ethz.ch/pipermail/bioconductor/2005-May/009027.html
>
>Thank you,
>
>Lucas
>
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Naomi S. Altman                                814-865-3791 (voice)
Associate Professor
Dept. of Statistics                              814-863-7114 (fax)
Penn State University                         814-865-1348 (Statistics)
University Park, PA 16802-2111