[BioC] problem with reading agilent microarray data into agi4x44preprocess
Jarek Bryk
bryk at evolbio.mpg.de
Mon May 18 18:13:38 CEST 2009
Hello,
I am trying to read my Agilent single color whole mouse genome
microarray data into Agi4x44PreProcess, as described in the vignette.
However, I get the error:
>
Error in readGenericHeader(fullname, columns = columns, sep = sep) :
Specified column headings not found in file
Following the advice from Martin Morgan (using options(error=recover)
etc., see
https://stat.ethz.ch/pipermail/bioconductor/2007-October/019577.html) I
figured out that the error is caused by the lack of the BGUsed column in
my raw data text file. And I don't know how to fix it. Is there a way of
telling the read.maimages/read.AgilentFE to skip this column (I can't
find this solution in the read.maimages help), or to modify the raw data
files so that the function doesn't complain (simply pasting the column
header does not work, I tried...)?
> sessionInfo()
R version 2.9.0 (2009-04-17)
i386-apple-darwin8.11.1
locale:
C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] mgug4122a.db_2.2.11 RSQLite_0.7-1 DBI_0.2-4
Agi4x44PreProcess_1.4.0 genefilter_1.24.0 annotate_1.22.0
AnnotationDbi_1.6.0 limma_2.18.0
[9] Biobase_2.4.1
loaded via a namespace (and not attached):
[1] splines_2.9.0 survival_2.35-4 tools_2.9.0 xtable_1.5-5
I would be very grateful for help,
cheers
jarek
--
Jarek Bryk
Max Planck Institute for Evolutionary Biology
August Thienemann Str. 2 | 24306 Plön, Germany
tel. +49 4522 763 287 | bryk at evolbio.mpg.de
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