[BioC] flowCore - cloud()

[Deepayan Sarkar]

On 1/29/09, Steve Lauriault <stevan at lauriault.com> wrote:
> Thank you Nishant,
>
>  Not to be nit-picky, but is there a function that would allow one to rotate
>  and/or zoom-in on the cube?  If not, there is a plot developed in MatLab by
>  Laszlo Balkay (sp?) that allows one to do this (rotate, zoom-in, etc) with
>  3-D FCS dataframes.  Not sure how congruent the two languages are, or if
>  this is any help at all, but I thought I'd relay the info.  I thought it was
>  a cool function, but it would be cooler if one could add colour to represent
>  fluorescence intensity, offspring or subset populations in the 4th
>  dimension.

The rgl package should be able to do all this fairly easily. There is
no convenience API predefined to allow this with flowFrames, but as
Nishant said, there is only one extra step needed:

1. extract the expressions from a flowFrame as a data frame or matrix

2. use the standard rgl API

There are many examples accompanying rgl that should get you started.

-Deepayan