[BioC] Affymetrix expression values using threestep?
Javier Pérez Florido
jpflorido at gmail.com
Wed Jan 21 19:40:47 CET 2009
Hi everyone,
I'm trying to get affymetrix expression values using three different
functions: expresso, mas5 and threestep.
Using expresso:
eset<- expresso(object, bgcorrect.method = "mas", pmcorrect.method =
"mas", normalize = FALSE, summary.method = "mas")
eset<-affy.scalevalue.exprSet(eset)
exprs(eset)<-log2(exprs(eset))
Using mas5
eset<-mas5(data)
exprs(eset)<-log2(exprs(eset))
But I have no idea how to get into this using threestep. I've tried:
eset<-threestep(data,background.method="MAS",normalize.method="scaling",summary.method="tukey.biweight")
But the results are quite different to the ones given by mas5 and
expresso (which are exactly the same between them). I know that
threestep returns log2 values...
Any tips??
Thanks in advance,
Javier
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