[BioC] RinGO problem
Jianping Jin
jjin at email.unc.edu
Mon Jan 12 18:05:59 CET 2009
Dear list,
I ran "runExonerate.sh" and had the per-chromosome output files condensed
into one single file (see below,
I read in the file just for viewing):
> str(allchranno)
'data.frame': 390388 obs. of 6 variables:
$ SEQ_ID : Factor w/ 388250 levels "chr1:10000013-10000070",..: 31687
28404 33240 34681 29011 26915 ...
$ PROBE_ID : Factor w/ 373478 levels "5313_0001_0001",..: 138251 325230
15265 268671 45500 270116 ...
$ CHROMOSOME: Factor w/ 21 levels "1","10","11",..: 2 2 2 2 2 2 2 2 2 2 ...
$ POSITION : int 75573476 4540877 79390517 80647222 5734395 30338873
82085749 7386228 61247293 ...
$ LENGTH : int 50 50 50 60 50 60 50 50 50 52 ...
$ MISMATCHES: int 0 0 0 0 0 0 0 0 0 0 ...
But when I tried to map probes to the genome I got an error and warnings:
probeAnno <-
posToProbeAnno("C:/from_DriveD/Chip-chip/Bultman/allChromExonerateOut_scott.txt")
Creating probeAnno mapping for chromosome 1 10 11 12 13 14 15 16 17 18 19 2
3 4 5 6 7 8 9 X Y Done.
Error in validObject(.Object) : invalid class "probeAnno" object: FALSE
In addition: Warning message:
In validityMethod(object) :
Some match positions end before they actually start.
Please check elements 1.start and 1.end .
Appreciate it if you can help!
Jianping
FYI:
> sessionInfo()
R version 2.8.0 (2008-10-20)
i386-pc-mingw32
locale:
LC_COLLATE=English_United States.1252;LC_CTYPE=English_United
States.1252;LC_MONETARY=English_United
States.1252;LC_NUMERIC=C;LC_TIME=English_United States.1252
attached base packages:
[1] splines tools stats graphics grDevices utils datasets
methods base
other attached packages:
[1] Ringo_1.6.0 SparseM_0.78 RColorBrewer_1.0-2 vsn_3.8.0
affy_1.20.0
[6] limma_2.16.3 geneplotter_1.20.0 annotate_1.20.1
xtable_1.5-4 AnnotationDbi_1.4.2
[11] lattice_0.17-15 genefilter_1.22.0 survival_2.34-1
Biobase_2.2.1
loaded via a namespace (and not attached):
[1] affyio_1.10.1 DBI_0.2-4 grid_2.8.0
KernSmooth_2.22-22 preprocessCore_1.4.0
[6] RSQLite_0.7-1
##################################
Jianping Jin Ph.D.
Bioinformatics scientist
Center for Bioinformatics
Room 3133 Bioinformatics building
CB# 7104
University of Chapel Hill
Chapel Hill, NC 27599
Phone: (919)843-6105
FAX: (919)843-3103
E-Mail: jjin at email.unc.edu
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