[BioC] justGCRMA problem :(
Keith Satterley
keith at wehi.EDU.AU
Wed Oct 29 01:46:45 CET 2008
Just thought I would test this on a Windows platform:
It reports:
Cel file C:/R/R-2.8.0/library/matchprobes/extdata/CL2001032020AA.cel does not
seem to have the correct dimensions.
Details below:
sessionInfo()
R version 2.8.0 (2008-10-20)
i386-pc-mingw32
locale:
LC_COLLATE=English_Australia.1252;LC_CTYPE=English_Australia.1252;LC_MONETARY=English_Australia.1252;LC_NUMERIC=C;LC_TIME=English_Australia.1252
attached base packages:
[1] splines tools stats graphics grDevices datasets utils
methods base
other attached packages:
[1] gcrma_2.14.0 matchprobes_1.14.0 affy_1.20.0 Biobase_2.2.0
loaded via a namespace (and not attached):
[1] affyio_1.10.0 preprocessCore_1.4.0
> celfile.path <- file.path("C:/R/R-2.8.0/library/matchprobes/extdata")
> celfile.path
[1] "C:/R/R-2.8.0/library/matchprobes/extdata"
> filenames <- list.celfiles(path=celfile.path, full.names=FALSE)
> filenames
[1] "118T1.cel" "CL2001032020AA.cel"
> normData <- justGCRMA(filenames=filenames, celfile.path=celfile.path,
type="affinities", fast=TRUE)
Computing affinities.Done.
Error in read.probematrix(filenames = filenames, which = "pm", cdfname =
cdfname, :
Cel file C:/R/R-2.8.0/library/matchprobes/extdata/CL2001032020AA.cel does not
seem to have the correct dimensions
>
maybe that helps pinpoint a problem.
cheers,
Keith
========================
Keith Satterley
Bioinformatics Division
The Walter and Eliza Hall Institute of Medical Research
Parkville, Melbourne,
Victoria, Australia
=======================
Patrick Aboyoun wrote:
> Cecile,
> I took a look at the gcrma package to see if this was a Mac OS
> X-specific issue and the best I can tell it is not. When I tried a naive
> application of the justGCRMA function to some cel files found in the
> matchprobes package when using a Linux machine, I got a segmentation
> fault that brought down R 2.8.0. I am cc'ing Jean(ZHIJIN) Wu, the gcrma
> package maintainer, to address this issue.
>
>
> > library(gcrma)
> Loading required package: Biobase
> Loading required package: tools
>
> Welcome to Bioconductor
>
> Vignettes contain introductory material. To view, type
> 'openVignette()'. To cite Bioconductor, see
> 'citation("Biobase")' and for packages 'citation(pkgname)'.
>
> Loading required package: affy
> Loading required package: matchprobes
> Loading required package: splines
> > sessionInfo()
> R version 2.8.0 (2008-10-20)
> x86_64-unknown-linux-gnu
>
> locale:
> LC_CTYPE=en_US;LC_NUMERIC=C;LC_TIME=en_US;LC_COLLATE=en_US;LC_MONETARY=C;LC_MESSAGES=en_US;LC_PAPER=en_US;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US;LC_IDENTIFICATION=C
>
>
> attached base packages:
> [1] splines tools stats graphics grDevices utils datasets
> [8] methods base
> other attached packages:
> [1] gcrma_2.14.0 matchprobes_1.14.0 affy_1.20.0
> Biobase_2.2.0
> loaded via a namespace (and not attached):
> [1] affyio_1.10.0 preprocessCore_1.4.0
> > celfile.path <- file.path("matchprobes/inst/extdata")
> > filenames <- list.celfiles(path=celfile.path, full.names=FALSE)
> > filenames
> [1] "118T1.cel" "CL2001032020AA.cel"
> > normData <- justGCRMA(filenames=filenames, celfile.path=celfile.path,
> type="affinities", fast=TRUE)
> Computing affinities.Done.
> Segmentation fault
>
>
>
> Cecile Laurent wrote:
>> Dear BioC,
>>
>> I've just updated R (2.8) and bioconductor (2.3), and justGCRMA
>> function doesn't work :
>>
>>
>> > sessionInfo()
>> R version 2.8.0 (2008-10-20)
>> powerpc-apple-darwin8.11.1
>>
>> locale:
>> C
>>
>> attached base packages:
>> [1] splines tools stats graphics grDevices utils datasets
>> [8] methods base other attached packages:
>> [1] gcrma_2.14.0 matchprobes_1.14.0 affy_1.20.0
>> Biobase_2.2.0 loaded via a namespace (and not attached):
>> [1] affyio_1.10.0 preprocessCore_1.4.0
>>
>>
>>
>> > require(gcrma)
>> > celfile.path <-
>> file.path("/Users/claurent/Documents/MU_Doc/Data/Transcriptome/CEL_Lignees/")
>>
>> > filenames <- list.celfiles(path=celfile.path, full.names=FALSE)
>> > normData <- justGCRMA(filenames=filenames,
>> celfile.path=celfile.path, type="affinities", fast=TRUE)
>> Computing affinities.Done.
>> Adjusting for optical effect..............Done.
>> Adjusting for non-specific binding.............Done.
>> Erreur dans just.gcrma(filenames = l$filenames, phenoData =
>> l$phenoData, :
>> INTEGER() can only be applied to a 'integer', not a 'environment'
>>
>>
>>
>>
>> Any idea of what is going wrong with the new version?
>>
>> _______________________________________________
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>
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