[BioC] Illumina annotation and lumi
Dan Swan
bioinformatics.lists at gmail.com
Wed Oct 15 12:59:57 CEST 2008
Hi Bioconductors,
following the lumi vignette I was hoping to analyse some IlluminaHuman
v3 data, but I'm curious about the resulting annotation - especially
with regards to the nuIDs.
briefly:
> library(lumi)
> fileName<-'raw_data.txt'
> x.lumi<-lumiR(fileName, lib='lumiHumanV2')
> lumi.N.Q <- lumiExpresso(x.lumi)
If I look at the resulting data:
iUYoe44olUvV11drzA 7.012843 7.022146 6.981181 7.002568 6.990872 7.009302
0006450661 8.052178 8.018692 8.250159 7.993246 7.944218 7.758490
i4et1wUK5HiCQ0Gra0 7.212793 7.148882 7.113698 7.192300 7.170937 7.034975
K1gAjeWlSJHV35XDXo 7.012843 7.043321 6.985838 7.084904 7.109322 7.048612
KXeDlyuUl4jKR6PFEo 7.110156 7.082396 7.129588 7.138857 7.147254 7.109566
unMWRFJdQ0ZgRIGERU 7.353679 7.364129 7.476052 7.395571 7.355086 7.230908
QBBnlu7edxepCdCh1c 7.011031 7.009087 6.998372 7.022854 6.983690 7.008475
0007510500 7.003781 7.003634 7.070783 6.998263 7.013868 6.958253
0004490414 7.034178 6.976611 7.011459 6.961637 7.065312 7.019079
HkrqGJVElV10AGFF1I 7.204322 7.197918 7.212510 7.023718 7.101016 7.161244
0004150270 7.251880 7.256996 7.260095 7.073517 7.111226 7.217829
KqgniRuCdnr3uYKs9c 7.587018 7.416183 7.402358 7.526966 7.198392 7.245086
> nuId<-"iUYoe44olUvV11drzA"
> nuID2targetID(nuId, lib = "lumiHumanV2")
$iUYoe44olUvV11drzA
[1] "ILMN_2722"
but... what are these 000 prefixed numbers?
> nuId<-"0006370768"
> nuID2targetID(nuId, lib = "lumiHumanV2")
$`0006370768`
character(0)
Session info below:
R version 2.7.1 (2008-06-23)
i386-apple-darwin8.10.1
locale:
en_GB.UTF-8/en_GB.UTF-8/C/C/en_GB.UTF-8/en_GB.UTF-8
attached base packages:
[1] tools stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] vsn_3.6.0 limma_2.14.6 lattice_0.17-8
[4] lumiHumanV2_1.3.1 lumi_1.6.2 mgcv_1.4-0
[7] affy_1.18.2 preprocessCore_1.2.1 affyio_1.8.1
[10] Biobase_2.0.1
loaded via a namespace (and not attached):
[1] grid_2.7.1
Thanks in advance!
regards,
Dan
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