[BioC] annotation information about ecoli2
James W. MacDonald
jmacdon at med.umich.edu
Thu Nov 6 16:14:47 CET 2008
Hi Minwook,
Minwook Kim wrote:
> Hi,
> I run the aafTableAnn() to get the annotation information of eocli2.
> But I got the error. I did try to get the ecoli package. but it failed. Anyone help me?
>> AnnTable2 <- aafTableAnn(AllGenes,chiptype,AnnCols[2]);
> Loading required package: ecoli2
> Error in aaf.handler(chip = chip) : Couldn't load data package ecoli2
> In addition: Warning message:
> In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, :
> there is no package called 'ecoli2'
This is true. In addition, there is no ecoli2.db package, nor can I find
any Biomart servers that might help. So for the time being, it appears
the best bet for annotation is the annotation file from Affymetrix.
http://www.affymetrix.com/Auth/analysis/downloads/na26/ivt/E_coli_2.na26.annot.csv.zip
You can make HTML or text tables using this file and the annotate
package if that is what you are trying to do - see the 'Get HTML Output'
vignette.
Best,
Jim
>
>
> Apparently, the following information are helpful to fix it.
>> chiptype
> [1] "ecoli2"
>
> "AllGenes" variable includes probe ids.
>
> I guess "ecoli2" microarry is not supported by annaffy package. or I don"t know how to download the ecoli2 package.
>
> Thanks.
> Minwook
>
>
>> sessionInfo()
> R version 2.7.0 (2008-04-22)
> x86_64-redhat-linux-gnu
> locale:
> LC_CTYPE=en_US.UTF-8;LC_NUMERIC=C;LC_TIME=en_US.UTF-8;LC_COLLATE=en_US.UTF-8;LC_MONETARY=C;LC_MESSAGES=en_US.UTF-8;LC_PAPER=en_US.UTF-8;LC_NAME=C;LC_ADDRESS=C;LC_TELEPHONE=C;LC_MEASUREMENT=en_US.UTF-8;LC_IDENTIFICATION=C
> attached base packages:
> [1] splines tools stats graphics grDevices utils datasets
> [8] methods base
> other attached packages:
> [1] gplots_2.6.0 gdata_2.4.2 gtools_2.5.0
> [4] simpleaffy_2.16.1 ecoli2probe_2.2.0 ecoli2cdf_2.2.0
> [7] bioDist_1.12.0 genefilter_1.20.1 survival_2.34-1
> [10] annaffy_1.12.1 KEGG.db_2.2.0 GO.db_2.2.0
> [13] AnnotationDbi_1.2.2 RSQLite_0.7-1 DBI_0.2-4
> [16] gcrma_2.12.1 matchprobes_1.12.1 affy_1.18.2
> [19] preprocessCore_1.2.1 affyio_1.8.1 Biobase_2.0.1
> loaded via a namespace (and not attached):
> [1] annotate_1.18.0
>
>
>
>
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--
James W. MacDonald, M.S.
Biostatistician
Hildebrandt Lab
8220D MSRB III
1150 W. Medical Center Drive
Ann Arbor MI 48109-0646
734-936-8662
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