[BioC] Error using Ringos readNimblegen
Morten Hansen
mhansen at imbg.ku.dk
Wed May 28 11:17:59 CEST 2008
I have a problem with the Ringo package.
I am trying to read in the results from a 1.5Kb Human Promoter Array
(CHP0150-HP1). I get the following error shown in below in the "Output"
section.
The target.txt, spottype.txt and sessionInfo() is shown below the
"Output" section.
**************
*** Output ***
**************
> myDir <- c("/home/mhansen/R/ChIP-chip/39037")
> myRg <- readNimblegen("targets.txt","spottypes.txt", path = myDir)
Reading targets file...
Reading raw intensities...
Read header information
Read /home/mhansen/R/ChIP-chip/39037/39037_532_pair.txt
Read /home/mhansen/R/ChIP-chip/39037/39037_635_pair.txt
Error in RG$R[, i] <- obj[, columns$f] :
number of items to replace is not a multiple of replacement length
>
The target.txt and spottype.txt look like this:
******************
*** target.txt ***
******************
SlideNumber FileNameCy3 FileNameCy5 Species Cy3 Cy5
HSAP0406 39037_532_pair.txt 39037_635_pair.txt Homo
sapiens (human) total HSAP0406
********************
*** spottype.txt ***
********************
SpotType GENE_EXPR_OPTION PROBE_ID Color
Probe BLOCK1 * black
********************
*** sessionInfo()***
********************
> sessionInfo()
R version 2.6.2 (2008-02-08)
x86_64-unknown-linux-gnu
locale:
...
attached base packages:
[1] tools stats graphics grDevices utils datasets
methods base
other attached packages:
[1] Ringo_1.2.0 RColorBrewer_1.0-2 vsn_3.2.1
affy_1.16.0 preprocessCore_1.0.0 affyio_1.6.1 limma_2.12.0
[8] geneplotter_1.16.0 lattice_0.17-8 annotate_1.16.1
xtable_1.5-2 AnnotationDbi_1.0.6 RSQLite_0.6-8
DBI_0.2-4 [15] Biobase_1.16.3
loaded via a namespace (and not attached):
[1] grid_2.6.2 KernSmooth_2.22-22 rcompgen_0.1-17
>
Any suggestions?
Morten Hansen MD
ICMM, Panum institute
University of Copenhagen
Email: mhansen at imbg.ku.dk
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