[BioC] Agilent dual channel treated as single channel

Sean Davis sdavis2 at mail.nih.gov
Sat Mar 29 23:28:09 CET 2008


On Sat, Mar 29, 2008 at 6:18 PM, Srinivas Iyyer
<srini_iyyer_bio at yahoo.com> wrote:
> Dear list,
>
>  can a 44x4 dual channel chip treated as single
>  channel. in such cases how to normalize using limma.
>  any suggestions please.

Hi, Srini.

There is a section of the limma user guide that addresses
between-array normalization of two-color arrays.  You might have a
look at that.  There is also a chapter about separate channel analysis
of two-color data.  If you have specific questions, you can always ask
here.

Sean



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