[BioC] probe file for custom array

[Lana Schaffer]

Jim,
I just wanted to get the sequence of the probes and the GC
content.  Looks like I don't need to use matchprobes and
just write/find the function to get the GC content.  I get
that matchprobes assumes MM probes exist.
Lana 

-----Original Message-----
From: James W. MacDonald [mailto:jmacdon at med.umich.edu] 
Sent: Monday, March 03, 2008 7:59 AM
To: Lana Schaffer
Cc: bioconductor at stat.math.ethz.ch
Subject: Re: [BioC] probe file for custom array

Hi Lana,

I doubt you can put in fake MM probes for the GC probes. Does this chip
really have MM probes, or does it have genomic and antigenomic probes
that are used instead of MM probes (of which I would assume the GC
probes are a part)?

There may be a way to hack things to work, but the best person to ask
would be Zhijin Wu, the maintainer of gcrma (If you in fact are
intending to use gcrma. Another assumption on my part since you don't
say what you are really trying to do.).

Anyway, I don't think faking things is the answer.

Best,

Jim



Lana Schaffer wrote:
> Jim,
> I think that is the problem.  There are both MM probes for the species

> and there are GC probes for background calculations.
> I was wondering if I could put in fake MM probes for the GC probes??
> Lana
> 
> -----Original Message-----
> From: James W. MacDonald [mailto:jmacdon at med.umich.edu]
> Sent: Friday, February 29, 2008 5:47 AM
> To: Lana Schaffer
> Cc: bioconductor at stat.math.ethz.ch
> Subject: Re: [BioC] probe file for custom array
> 
> Hi Lana,
> 
> Is this a PM-only chip?
> 
> Jim
> 
> 
> 
> Lana Schaffer wrote:
>> Jim,
>> I have installed the custom CDF package and still not able to finish 
>> creating the probe package.  This is my error:
>> Importing the data.
>> Error in rep(NA, max(pm1, mm1, pm2, mm2)) : invalid 'times' argument 
>> Can you give me a hint to figure out the problem?
>>
>>> filename <- system.file("extdata",
>> "GLYCOv3_Hs_probeseqs.gz",package="matchprobes")
>>> outdir <- tempdir()
>>> me <- "Lana Schaffer <schaffer at scripps.edu>"
>>> species <- "homo_sapiens"
>>> makeProbePackage("glycov3Hs", datafile=gzfile(filename, open="r"),
>> + outdir=outdir,maintainer=me,species=species, version =
>> "0.0.1",force=TRUE)
>> Importing the data.
>> Error in rep(NA, max(pm1, mm1, pm2, mm2)) : invalid 'times' argument
> 
>>> sessionInfo()
>> R version 2.6.0 (2007-10-03)
>> i386-pc-mingw32
>>
>> other attached packages:
>>  [1] glycov3hscdf_1.16.0  makecdfenv_1.16.0    simpleaffy_2.14.05  
>>  [4] genefilter_1.16.0    survival_2.32        affyPLM_1.14.0      
>>  [7] gcrma_2.10.0         matchprobes_1.10.0   affy_1.16.0         
>> [10] preprocessCore_1.0.0 affyio_1.6.0         Biobase_1.16.3      
>>
>>    Lana
>> -----Original Message-----
>> From: James MacDonald [mailto:jmacdon at med.umich.edu]
>> Sent: Tuesday, February 26, 2008 3:39 PM
>> To: Lana Schaffer
>> Cc: bioconductor at stat.math.ethz.ch
>> Subject: Re: [BioC] probe file for custom array
>>
>> You get the probe_tab file from Affymetrix and then use
>> makeProbePackage() in the matchprobes package.
>>
>> Best,
>>
>> Jim
>>
>>
>>
>> Lana Schaffer wrote:
>>> Hi,
>>> I would like to make a probe file for a custom array.  How do I do 
>>> this?
>>>
>>> Lana Schaffer
>>> Biostatistics/Informatics
>>> The Scripps Research Institute
>>> DNA Array Core Facility
>>> La Jolla, CA 92037
>>> (858) 784-2263
>>> (858) 784-2994
>>> schaffer at scripps.edu
>>>
>>> _______________________________________________
>>> Bioconductor mailing list
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>> --
>> James W. MacDonald, MS
>> Biostatistician
>> UMCCC cDNA and Affymetrix Core
>> University of Michigan
>> 1500 E Medical Center Drive
>> 7410 CCGC
>> Ann Arbor MI 48109
>> 734-647-5623
> 
> --
> James W. MacDonald, M.S.
> Biostatistician
> Affymetrix and cDNA Microarray Core
> University of Michigan Cancer Center
> 1500 E. Medical Center Drive
> 7410 CCGC
> Ann Arbor MI 48109
> 734-647-5623

--
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623