[BioC] flowCore - how to see the marker names?
Nolwenn LeMeur
nlemeur at fhcrc.org
Wed Jun 11 15:06:15 CEST 2008
Hi Anne,
The parameter annotation of a flowframe is stored in an annotated
dataframe. You can access the information using the pData function:
pData(parameters(FCS))
see the flowFrame-class man page for more details
Nolwenn
Anne wrote:
>Hi,
>
>I am having problems in finding the marker names in a flowFrame.
>(The marker names mapped onto the channel names - i.e.FL1-H, FL2-H, FL3-H
>and FL4-H).
>
>I wrote the code:
>
>FCS <- read.FCS(subfilesdir2)
>FCS
>featureNames(FCS)
>
>and got this output - but it does not seem to show any new names (i.e CD45),
>so am I doing the right thing:
>
>flowFrame object 'P:/Projekter/Scientific Projects/AML_subgroup specific
>gene expression_2008/Part A/Data/Raa
>data/AML_FC_Herlev/de39patienter/BH13100/BH13100.002'
>with 100000 cells and 7 observables:
> name desc range minRange maxRange
>$P1 FSC-H FSC-Height 1024 0 1023
>$P2 SSC-H SSC-Height 1024 0 1023
>$P3 FL1-H FL1-Height 1024 1 10000
>$P4 FL2-H FL2-Height 1024 1 10000
>$P5 FL3-H FL3-Height 1024 1 10000
>$P6 FL1-A <NA> 1024 0 1023
>$P7 FL4-H FL4-Height 1024 1 10000
>132 keywords are stored in the 'descripton' slot
>
>
>>featureNames(FCS)
>>
>>
> $P1N $P2N $P3N $P4N $P5N $P6N $P7N
>"FSC-H" "SSC-H" "FL1-H" "FL2-H" "FL3-H" "FL1-A" "FL4-H"
>
>Thanks in advance
>
>/Anne
>
>
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>
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