[BioC] DAT to CEL
Henrik Bengtsson
hb at stat.berkeley.edu
Tue Jan 15 17:19:58 CET 2008
See also the recent:
@ARTICLE{ArteagaSalasJ_etal_2008,
author = {Jose M Arteaga-Salas and Harry Zuzan and William B Langdon
and Graham
J G Upton and Andrew P Harrison},
title = {An overview of image-processing methods for Affymetrix GeneChips.},
journal = {Brief Bioinform},
year = {2008},
volume = {9},
pages = {25--33},
number = {1},
month = {Jan},
abstract = {We present an overview of image-processing methods for Affymetrix
GeneChips. All GeneChips are affected to some extent by spatially
coherent defects and image processing has a number of potential impacts
on the downstream analysis of GeneChip data. Fortunately, there are
now a number of robust and accurate algorithms, which identify the
most disabling defects. One group of algorithms concentrate on the
transformation from the original hybridisation DAT image to the representative
CEL file. Another set uses dedicated pattern recognition routines
to detect different types of hybridisation defect in replicates.
A third type exploits the information provided by public repositories
of GeneChips (such as GEO). The use of these algorithms improves
the sensitivity of GeneChips, and should be a prerequisite for studies
in which there are only few probes per relevant biological signal,
such as exon arrays and SNP chips.},
doi = {10.1093/bib/bbm055},
pii = {bbm055},
pmid = {18057073},
timestamp = {2008.01.15},
url = {http://dx.doi.org/10.1093/bib/bbm055}
}
/Henrik
On 15/01/2008, Kevin R. Coombes <krcoombes at mdacc.tmc.edu> wrote:
> If you go to our course web site:
> http://bioinformatics.mdanderson.org/MicroarrayCourse/Lectures07/
> and check out Lecture 1, there is a description of how DAT files are
> turned into CEL files.
> Kevin
>
> BURCIN BEKTAS wrote:
> >
> > Greetings,
> >
> > Does anybody know how affymetrix GCOS converts DAT files to CEL files?
> > Gridding, feature extraction (with coefficient of variance update) etc.
> > is being done but are there any reference documents that fully explain
> > this procedure or is this a closed information?
> >
> > Thanks
> >
> >
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