[BioC] DAT to CEL

Henrik Bengtsson hb at stat.berkeley.edu
Tue Jan 15 17:19:58 CET 2008


See also the recent:

@ARTICLE{ArteagaSalasJ_etal_2008,
  author = {Jose M Arteaga-Salas and Harry Zuzan and William B Langdon
and Graham
	J G Upton and Andrew P Harrison},
  title = {An overview of image-processing methods for Affymetrix GeneChips.},
  journal = {Brief Bioinform},
  year = {2008},
  volume = {9},
  pages = {25--33},
  number = {1},
  month = {Jan},
  abstract = {We present an overview of image-processing methods for Affymetrix
	GeneChips. All GeneChips are affected to some extent by spatially
	coherent defects and image processing has a number of potential impacts
	on the downstream analysis of GeneChip data. Fortunately, there are
	now a number of robust and accurate algorithms, which identify the
	most disabling defects. One group of algorithms concentrate on the
	transformation from the original hybridisation DAT image to the representative
	CEL file. Another set uses dedicated pattern recognition routines
	to detect different types of hybridisation defect in replicates.
	A third type exploits the information provided by public repositories
	of GeneChips (such as GEO). The use of these algorithms improves
	the sensitivity of GeneChips, and should be a prerequisite for studies
	in which there are only few probes per relevant biological signal,
	such as exon arrays and SNP chips.},
  doi = {10.1093/bib/bbm055},
  pii = {bbm055},
  pmid = {18057073},
  timestamp = {2008.01.15},
  url = {http://dx.doi.org/10.1093/bib/bbm055}
}

/Henrik

On 15/01/2008, Kevin R. Coombes <krcoombes at mdacc.tmc.edu> wrote:
> If you go to our course web site:
>      http://bioinformatics.mdanderson.org/MicroarrayCourse/Lectures07/
> and check out Lecture 1, there is a description of how DAT files are
> turned into CEL files.
>         Kevin
>
> BURCIN BEKTAS wrote:
> >
> > Greetings,
> >
> > Does anybody know how affymetrix GCOS converts DAT files to CEL files?
> > Gridding, feature extraction (with coefficient of variance update) etc.
> > is being done but are there  any reference documents that fully explain
> > this procedure or is this a closed information?
> >
> > Thanks
> >
> >
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