[BioC] row.names in the latest vsn
Roumyana Yordanova
roumyana.yordanova at bms.com
Thu Apr 3 15:29:25 CEST 2008
Dear Wolfgang,
Thanks a lot for your help.
Roumyana
Wolfgang Huber wrote:
> Dear Roumyana,
>
> this is because I rationalized the function structure, as follows:
>
> 1.) The generic function "vsn2" has methods for data objects of class
> AffyBatch, ExpressionSet, NChannelSet, RGList and matrix. It returns a
> fit object of class "vsn" with model fit parameters etc. The generic
> function "predict" applies a fitted model to a data object (of any of
> the above classes) and, in the case of AffyBatch and in most other
> cases, returns a data object of the same class and shape.
>
> 2.) So "justvsn" is now literally just as follows:
>
> justvsn = function(x, ...) {
> fit = vsn2(x, ...)
> predict(fit, newdata=x, useDataInFit=TRUE)
> }
>
> 3.) If you also want to do the probe-set summarization by RMA (an
> Affymetrix-specific feature that has nothing directly to do with vsn),
> you can call
>
> vsnrma
>
> which is a wrapper around "justvsn" and then "rma" from the affy
> package where the latter is called with options normalize=FALSE,
> background=FALSE.
>
> Bottomline: please have a look at the vsnrma function.
>
> Also, for all those of you who have endured the vsn version 1 -->
> version 2 transition, to all intents and purposes the reengineering of
> vsn is now done and I intend to make no further changes to the user
> interface.
>
> Best wishes
> Wolfgang
>
> ------------------------------------------------------------------
> Wolfgang Huber EBI/EMBL Cambridge UK http://www.ebi.ac.uk/huber
>
> Roumyana Yordanova wrote:
>
>> Dear Wolfgang,
>>
>> It seems that justvsn in the last version
>> (http://www.bioconductor.org/packages/devel/bioc/html/vsn.html) does
>> not output the row.names (the corresponding probe sets names). Can
>> this be something related to changes in the AffyBatch object?
>>
>> Roumyana
>>
>> Roumyana Yordanova wrote:
>>
>>> Dear Wolfgang,
>>> Thanks for your help. For anyone that applies vsn2 to AffyBatch
>>> objects with a reference set please use the last version of vsn
>>> http://www.bioconductor.org/packages/devel/bioc/html/vsn.html
>>> Roumyana
>>>
>>>
>>>
>>> ------------------------------------------------------------------------
>>>
>>>
>>> Subject:
>>> Re: vsn with a reference set
>>> From:
>>> Wolfgang Huber <huber at ebi.ac.uk>
>>> Date:
>>> Fri, 28 Mar 2008 12:05:40 +0000
>>> To:
>>> Roumyana Yordanova <roumyana.yordanova at bms.com>
>>>
>>> To:
>>> Roumyana Yordanova <roumyana.yordanova at bms.com>
>>>
>>>
>>> 28/03/2008 04:55 Roumyana Yordanova a écrit
>>>
>>>
>>>> Dear Wolfgang,
>>>> Thanks a lot. I got the current version and it works.
>>>> Roumyana
>>>>
>>>>
>>>
>>>
>>> Dear Roumyana
>>>
>>> thank you! Could you post this reply to the mailing list, so that
>>> everybody knows that they should upgrade their vsn if they want to work
>>> with reference normalisation and AffyBatch objects?
>>>
>>>
>>>
>>>
>>
>>
>
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