[BioC] limma design matrix for two color arrays with common reference
zones
zones at salk.edu
Fri Nov 30 02:36:51 CET 2007
Hello,
I am confused about how my design matrix should look. I have data
from 14 arrays, each with the same common reference in one channel
and a sample of interest in the other. There are technical and
biological replicates of each sample with wild type and two different
mutants as follows:
# green red
1 ref wt-A
2 ref wt-A
3 ref wt-B
4 ref wt-B
5 ref wt-C
6 ref wt-C
7 ref mut1-A
8 ref mut1-A
9 ref mut1-B
10 ref mut1-B
11 ref mut2-A
12 ref mut2-A
13 ref mut2-B
14 ref mut2-B
I wish to use duplicateCorrelation for the replicate arrays and then
ask what genes are differentially expressed in each mutant relative
to wild type. Any help is appreciated.
Thanks in advance!
Matt
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