[BioC] Illumina beadlevel expression data

Ina Hoeschele inah at vt.edu
Fri Nov 16 00:21:12 CET 2007


Thanks for writing, Wei. Sorry about that, I did get the wrong number. 
For the total number of beads I get length(BLData[[1]][,1])= 1,035,907. 
Please let me know if this does not look right.
Ina


Wei Shi wrote:
> Hi Ina:
>
>     It surprised me that you only got around 320,000 beads. You should 
> see at least 700,000 beads for each strip since there are about 24,000 
> bead types in each strip and each bead type have 30 or more beads. And 
> we actually saw about 1 million beads for each strip for our data. I 
> suspect you did not retrieve bead level data correctly.
>
> Cheers,
> Wei
>
> Ina Hoeschele wrote:
>> I have a total of 319952 beads. So I printed the information for the 
>> first 10 and for 10 others towards the end. Even for the first 10 beads 
>> the GrnX and GrnY are not negative. Here it is.
>>   
>>>  BLData[[1]][1:10,]
>>>     
>>    ProbeID          G Gb      GrnX      GrnY
>> 1    10008  0.0000000  0 1556.7592 13526.200
>> 2    10008  2.5249867  0 1713.2862 17711.700
>> 3    10008 -0.0359710  0   45.9317  6676.658
>> 4    10008  0.0000000  0 1401.8142  2153.181
>> 5    10008  0.0000000  0 2078.2252 15974.100
>> 6    10008 -6.8111267  0 1193.8842  8654.250
>> 7    10008  0.0000000  0   45.0026 13990.970
>> 8    10008 77.2345222  0  542.1487 14834.780
>> 9    10008 -0.8237067  0 1341.1462 13791.170
>> 10   10008 -0.2324444  0 1312.7072  7549.003
>>   
>>>  BLData[[1]][300000:300010,]
>>>     
>>        ProbeID           G Gb      GrnX      GrnY
>> 300000 2140131  0.00000000  0 1258.9752 17301.580
>> 300001 2140131 -0.24859133  0  398.1137  6108.199
>> 300002 2140131 -0.07388444  0 2094.6222 16298.300
>> 300003 2140131  0.00000000  0  642.9227  2094.740
>> 300004 2140131 11.67171933  0 1352.2322  6294.191
>> 300005 2140131  0.00000000  0 1104.5312 14069.740
>> 300006 2140131 -0.15372500  0  963.8952  9653.909
>> 300007 2140131  0.00000000  0  161.5619 14317.910
>> 300008 2140131 -0.08331556  0 1515.5492 13932.460
>> 300009 2140131  0.61296444  0 1934.0542 15118.710
>> 300010 2140131  0.00000000  0  177.0217 16717.080
>>
>>
>>



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