[BioC] Illumina beadlevel expression data
Ina Hoeschele
inah at vt.edu
Fri Nov 16 00:21:12 CET 2007
Thanks for writing, Wei. Sorry about that, I did get the wrong number.
For the total number of beads I get length(BLData[[1]][,1])= 1,035,907.
Please let me know if this does not look right.
Ina
Wei Shi wrote:
> Hi Ina:
>
> It surprised me that you only got around 320,000 beads. You should
> see at least 700,000 beads for each strip since there are about 24,000
> bead types in each strip and each bead type have 30 or more beads. And
> we actually saw about 1 million beads for each strip for our data. I
> suspect you did not retrieve bead level data correctly.
>
> Cheers,
> Wei
>
> Ina Hoeschele wrote:
>> I have a total of 319952 beads. So I printed the information for the
>> first 10 and for 10 others towards the end. Even for the first 10 beads
>> the GrnX and GrnY are not negative. Here it is.
>>
>>> BLData[[1]][1:10,]
>>>
>> ProbeID G Gb GrnX GrnY
>> 1 10008 0.0000000 0 1556.7592 13526.200
>> 2 10008 2.5249867 0 1713.2862 17711.700
>> 3 10008 -0.0359710 0 45.9317 6676.658
>> 4 10008 0.0000000 0 1401.8142 2153.181
>> 5 10008 0.0000000 0 2078.2252 15974.100
>> 6 10008 -6.8111267 0 1193.8842 8654.250
>> 7 10008 0.0000000 0 45.0026 13990.970
>> 8 10008 77.2345222 0 542.1487 14834.780
>> 9 10008 -0.8237067 0 1341.1462 13791.170
>> 10 10008 -0.2324444 0 1312.7072 7549.003
>>
>>> BLData[[1]][300000:300010,]
>>>
>> ProbeID G Gb GrnX GrnY
>> 300000 2140131 0.00000000 0 1258.9752 17301.580
>> 300001 2140131 -0.24859133 0 398.1137 6108.199
>> 300002 2140131 -0.07388444 0 2094.6222 16298.300
>> 300003 2140131 0.00000000 0 642.9227 2094.740
>> 300004 2140131 11.67171933 0 1352.2322 6294.191
>> 300005 2140131 0.00000000 0 1104.5312 14069.740
>> 300006 2140131 -0.15372500 0 963.8952 9653.909
>> 300007 2140131 0.00000000 0 161.5619 14317.910
>> 300008 2140131 -0.08331556 0 1515.5492 13932.460
>> 300009 2140131 0.61296444 0 1934.0542 15118.710
>> 300010 2140131 0.00000000 0 177.0217 16717.080
>>
>>
>>
More information about the Bioconductor
mailing list