[BioC] error with timecourse package
Ana Conesa
aconesa at ivia.es
Mon Mar 26 15:37:26 CEST 2007
I am trying to use the timecourse package to analyze gene expression
data in a multiseries experiment with the following design:
3 time points (6,24,48 hrs)
5 biological conditions (1 to 5)
2665 genes
2 biological replicates per condition expect for the last one that has
6 replicates. (individuals 1 to 14)
In total I have 42 slides, thus the dimensionality of my data matrix
is 2665x42
I create the different parameters for the mb.MANOVA function
> size2 <- matrix(rep(c(2,2,2,2,6),2665), ncol=5, byrow=T)
> assay2
[1] "rep1" "rep1" "rep1" "rep2" "rep2" "rep2" "rep3" "rep3"
"rep3" "rep4" "rep4" "rep4" "rep5" "rep6" "rep7" "rep5" "rep6"
[18] "rep7" "rep5" "rep6" "rep7" "rep8" "rep8" "rep8" "rep9"
"rep9" "rep9" "rep10" "rep10" "rep10" "rep11" "rep11" "rep11"
"rep12"
[35] "rep13" "rep14" "rep12" "rep13" "rep14" "rep12" "rep13" "rep14"
>trt2
[1] 1 1 1 2 2 2 3 3 3 4 4 4 5 5 5 5 5 5 5 5 5 1 1 1 2 2 2 3 3 3 4 4 4
5 5 5 5 5 5 5 5 5
> time2
[1] 6 24 48 6 24 48 6 24 48 6 24 48 6 6 6 24 24 24 48 48 48 6
24 48 6 24 48 6 24 48 6 24 48 6 6 6 24 24 24 48 48
When I execute the mb.MANOVA, I get the following error:
> result <- mb.MANOVA(data42, times=3, D=5, size=size2,
rep.grp=assay2, condition.grp=trt2, time.grp=time2)
Error in squeezeVar(diagSp[x, ], N - D) : lengths differ
Any idea what I am doing wrong and how to fix it??
Thanks!
Ana
O @@@@@ Ana Conesa, PhD.
@@@ O @@ O @ Centro de Genómica
@ O @@@@ O @ Instituto Valenciano de Investigaciones Agrarias
(IVIA)
@@@ O @@@@ Carretera Moncada - Naquera, Km. 4,5
@@@@ O @ 46113 Moncada (Valencia) SPAIN
|| Tel. +34 963424000 ext.70161; Fax. +34 963424001
|| email: aconesa at ivia.es
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