[BioC] MBEI on 60 U133 Plus 2.0 arrays
James MacDonald
jmacdon at med.umich.edu
Tue Mar 6 19:49:39 CET 2007
Hi Kamila,
Currently you are limited by the amount of RAM you have. Technically you _could_ write a just.li.wong() that bypasses the explicit creation of an AffyBatch (as do justRMA() and justGCRMA()), and quite possibly you would be able to get away with just 2Gb RAM.
Best,
Jim
--
James W. MacDonald, M.S.
Biostatistician
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
7410 CCGC
Ann Arbor MI 48109
734-647-5623
>>> On 3/6/2007 at 12:32 PM, <knaxerov at ix.urz.uni-heidelberg.de> wrote:
> Dear list,
>
> I am wondering if I have any other normalization options besides
> justRMA() and justGCRMA() if I want to normalize 60 U133 Plus 2.0
> arrays on a machine with 2 GB of RAM. Since I run out of memory when
> using ReadAffy on as few as 15 arrays, I suppose there really is no
> way to use MBEI, for example, correct?
>
> Thanks.
> Kamila
>
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