[BioC] hgu133a and gnGNF1Ba

Robert Castelo robert.castelo at upf.edu
Mon Jul 2 12:47:54 CEST 2007


hi,

i've downloaded the gene expression atlas data for human and mouse from
GEO

wget
ftp://ftp.ncbi.nih.gov/pub/geo/DATA/supplementary/series/GSE1133/GSE1133_RAW.tar

which as far as i know comes from the Affymetrix microarray chips
hgu133a.

i want to load and process myself these data using RMA in bioconductor.
after putting the files in the proper directory structure i do the
following:

library(affy)
library(hgu133a)

data <- ReadAffy()
eset <- rma(data)
Error in getCdfInfo(object) : Could not obtain CDF environment, problems
encountered:
Specified environment does not contain gnGNF1Ba
Library - package gngnf1bacdf not installed
Data for package affy did not contain gngnf1bacdf
Bioconductor - gngnf1bacdf not available

and i get the problem above, i'm missing a CDF package called
gngnf1bacdf. in fact this is the annotation that seems to be attached to
the .CEL files

annotation(data)
[1] "gngnf1ba"
print(data at cdfName)
[1] "gnGNF1Ba"

so i tried to download it from the bioconductor site:

source("http://www.bioconductor.org/getBioC.R")
getBioC(c("gngnf1bacdf"))
Running biocinstall version 2.0.8 with R version 2.5.0 
Your version of R requires version 2.0 of Bioconductor.
Warning in install.packages(pkgs = pkgs, repos = repos, dependencies =
dependencies,  : 
         argument 'lib' is missing: using '/home/robert/myRlibrary/'
Warning message:
package 'gngnf1bacdf' is not available in: install.packages(pkgs = pkgs,
repos = repos, dependencies = dependencies,  

but as you can see it does not seem to be available. does anyone know
where can i get this environment CDF package?

this is may sessionInfo():

R version 2.5.0 (2007-04-23) 
x86_64-unknown-linux-gnu 

locale:
C

attached base packages:
[1] "tools"     "stats"     "graphics"  "grDevices" "utils"
"datasets" 
[7] "methods"   "base"     

other attached packages:
      affy    Biobase hgu133acdf    hgu133a     affyio 
  "1.14.0"   "1.14.0"   "1.16.0"   "1.16.0"    "1.4.0" 


thanks!!
robert.



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