[BioC] problem with biomaRt getHomolog function

[Steffen Durinck]

Hi Mark,

I think the rat entrezgene id 613226 is a recently added entrezgene id 
and is not yet available in Ensembl.  Ensembl updates every two months 
and the last update of entrezgene id 613226 appears to be December 26 of 
2006.  So this might be the reason.
Also I would suggest you use the developmental version of biomaRt 
(biomaRt_1.9.15) to do getHomolog queries.  A recent change in the 
BioMart suite enables the biomaRt package to retrieve both the id you 
use to query and the ids in the result of the query. 

Here's an example:

rat.entrezgene.ID=c("24842","83502","24205")
mouse.mart <- useMart("ensembl","mmusculus_gene_ensembl")
rat.mart<- useMart("ensembl", "rnorvegicus_gene_ensembl")
mouse.homolog<-getHomolog(id =rat.entrezgene.ID, from.mart = 
rat.mart,from.type = "entrezgene",to.type="entrezgene", to.mart=mouse.mart)

 > mouse.homolog
     V1    V2
1 24842 22059
2 24205 11789
3 24205    NA
4 83502 12550

best,
Steffen

Kimpel, Mark William wrote:
> I am trying to use the getHomolog function of package "biomaRt" to map
> rat entrezgene IDs to mouse entrezgene IDs. For every ID I try, I get
> NULL as return, even when I know that a mouse mapping exists.
>
>  
>
> For example, ratID "613226" corresponds to mouse "229706" .
>
>  
>
> See my code and sessionInfo below. Anyone know what I am doing wrong?
>
>  
>
> Thanks, Mark
>
>  
>
>   
>> require(DBI)
>>     
>
> [1] TRUE
>
>   
>> require(biomaRt)
>>     
>
> [1] TRUE
>
>   
>
>   
>> mouse.mart <- useMart("ensembl","mmusculus_gene_ensembl")
>>     
>
> Checking attributes and filters ... ok
>
>   
>
>   
>> rat.mart<- useMart("ensembl", "rnorvegicus_gene_ensembl")
>>     
>
> Checking attributes and filters ... ok
>
>   
>
>   
>> rat.entrezgene.ID<-"613226"
>>     
>
>   
>>     
>>     
>
>   
>> mouse.homolog<-getHomolog(id =rat.entrezgene.ID, from.mart = rat.mart,
>>     
> from.type = "entrezgene", 
>
> +     to.type="entrezgene", to.mart=mouse.mart)
>
> Warning message:
>
> getBM returns NULL. in: getHomolog(id = rat.entrezgene.ID, from.mart =
> rat.mart, from.type = "entrezgene",  
>
>   
>
>  
>
>   
>> sessionInfo()
>>     
>
> R version 2.4.1 (2006-12-18) 
>
> i386-pc-mingw32 
>
>  
>
> locale:
>
> LC_COLLATE=English_United States.1252;LC_CTYPE=English_United
> States.1252;LC_MONETARY=English_United
> States.1252;LC_NUMERIC=C;LC_TIME=English_United States.1252
>
>  
>
> attached base packages:
>
> [1] "stats"     "graphics"  "grDevices" "datasets"  "utils"     "tools"
> "methods"   "base"     
>
>  
>
> other attached packages:
>
>  biomaRt    RCurl      XML      DBI  RWinEdt    limma     affy   affyio
> Biobase 
>
>  "1.8.1"  "0.8-0"  "1.2-0" "0.1-12"  "1.7-5"  "2.9.1" "1.12.2"  "1.2.0"
> "1.12.2"
>
>  
>
> Mark W. Kimpel MD 
>
>  
>
>  
>
> Official Business Address:
>
>  
>
> Department of Psychiatry
>
> Indiana University School of Medicine
>
> PR M116
>
> Institute of Psychiatric Research
>
> 791 Union Drive
>
> Indianapolis, IN 46202
>
>  
>
> Preferred Mailing Address:
>
>  
>
> 15032 Hunter Court
>
> Westfield, IN  46074
>
>  
>
> (317) 490-5129 Work, & Mobile
>
>  
>
> (317) 663-0513 Home (no voice mail please)
>
> 1-(317)-536-2730 FAX
>
>
> 	[[alternative HTML version deleted]]
>
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>   


-- 
Steffen Durinck, Ph.D.

Oncogenomics Section
Pediatric Oncology Branch
National Cancer Institute, National Institutes of Health
URL: http://home.ccr.cancer.gov/oncology/oncogenomics/

Phone: 301-402-8103
Address:
Advanced Technology Center,
8717 Grovemont Circle
Gaithersburg, MD 20877