[BioC] Advice on how to compare two different sources on cDNA
Marcelo Laia
marcelolaia at gmail.com
Wed Jan 24 12:52:12 CET 2007
Hi,
I would like to compare 2 RNA sources to see if a culture medium
mimetic the plant host.
There are evidence that a culture medium mimetic the plant, then I
inoculated the phytopatogenic bacteria in both, mimetic culture medium
and plant. After 24 hours, I was collected the RNA and did a 3
hibridizations:
Cy3 Cy5
mimetic culture medium vs NA
mimetic culture medium vs NA
mimetic culture medium vs NA
Cy3 Cy5
plant vs NA
plant vs NA
plant vs NA
NA = poor culture medium
I have hybridized they separated because I will use it in a time
course experiment.
I thinking to do a contrast like limma usersguide (11.5 Weaver Mutant
Data: A 2x2 Factorial Experiment).
If the mimetic medium is true (mimetic the plant) I don't will get any
differentially expressed genes. This aproach is good or you suggest me
other?
Thank you very much
--
Marcelo Luiz de Laia
Ph.D Candidate
São Paulo State University (http://www.unesp.br/eng/)
School of Agricultural and Veterinary Sciences
Department of Technology
Via de Acesso Prof. Paulo Donato Castellane s/n
14884-900 Jaboticabal - SP - Brazil
Phone: +55-016-3209-2675
Cell: +55-016-97098526
More information about the Bioconductor
mailing list