[BioC] using simpleaffy incorrectly, or is there a problem with the read.affy function?
k. brand
k.brand at erasmusmc.nl
Thu Jan 18 20:50:26 CET 2007
Biocers,
Updating to the latest simpleaffy- 2.8.0- solved the problem...
thanks again,
karl
on 1/18/2007 7:25 PM k. brand said the following:
> Dear BioCers,
>
> After setting my working directory and loading simpleaffy, i fail to
> read in my .CEL files using:
>
> rawAandB <- read.affy()
>
> Error in file(file, "r") : unable to open connection
> In addition: Warning message:
> cannot open file './covdesc', reason 'No such file or directory'
>
> And using:
>
> rawAandB <- read.affy("covdesc.txt")
> Error in function (classes, fdef, mtable) :
> unable to find an inherited method for function "pData", for
> signature "phenoData"
>
> where my "covdesc.txt" file in the wd looks like this:
>
> region
> Tco1A.CEL one
> Tco2A.CEL one
> Tco3B.CEL one
> Tco4B.CEL one
> Tmi1A.CEL two
> Tmi2A.CEL two
> Tmi3B.CEL two
> Tmi4B.CEL two
> Tsh1A.CEL three
> Tsh2A.CEL three
> Tsh3B.CEL three
> Tsh4B.CEL three
>
> Session Info and other console details as below.
>
> Using "library(affy)" and "rawAandB <- ReadAffy()" i have no problems.
>
> So am i using simpleaffy incorrectly, or is there a problem with the
> read.affy function??
>
>
>
> > library(simpleaffy)
> Loading required package: affy
> Loading required package: Biobase
> Loading required package: tools
>
> Welcome to Bioconductor
>
> Vignettes contain introductory material. To view, type
> 'openVignette()' or start with 'help(Biobase)'. For details
> on reading vignettes, see the openVignette help page.
>
> Loading required package: affyio
> Loading required package: genefilter
> Loading required package: survival
> Loading required package: splines
> Welcome to 'simpleaffy' V 2.4.0
> Produced by The Paterson Institute for Cancer Research
> and funded by CANCER RESEARCH UK.
> http://bioinformatics.picr.man.ac.uk/simpleaffy
> mailto: microarray at picr.man.ac.uk
> > ?read.affy
> > rawAandB <- read.affy()
> Error in file(file, "r") : unable to open connection
> In addition: Warning message:
> cannot open file './covdesc', reason 'No such file or directory'
> > rawAandB <- read.affy("covdesc.txt")
> Error in function (classes, fdef, mtable) :
> unable to find an inherited method for function "pData", for
> signature "phenoData"
>
> > sessionInfo()
> R version 2.4.0 (2006-10-03)
> i386-pc-mingw32
>
> locale:
> LC_COLLATE=English_United States.1252;LC_CTYPE=English_United
> States.1252;LC_MONETARY=English_United
> States.1252;LC_NUMERIC=C;LC_TIME=English_United States.1252
>
> attached base packages:
> [1] "splines" "tools" "methods" "stats" "graphics"
> "grDevices"
> [7] "utils" "datasets" "tcltk" "base"
>
> other attached packages:
> simpleaffy genefilter survival affy affyio Biobase
> svIO
> "2.4.2" "1.12.0" "2.30" "1.12.0" "1.2.0" "1.12.0"
> "0.9-5"
> R2HTML svMisc svSocket svIDE
> "1.58" "0.9-5" "0.9-5" "0.9-5"
> >
>
--
Karl Brand <k.brand at erasmusmc.nl>
Department of Genetics
Erasmus MC
Dr Molewaterplein 50
3015 GE Rotterdam
lab +31 (0)10 408 7409 fax +31 (0)10 408 9468
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