[BioC] Agilent 4100a probe annotation information
Daniel Brewer
daniel.brewer at icr.ac.uk
Mon Jan 8 14:50:19 CET 2007
Sean Davis wrote:
> On Monday 08 January 2007 07:24, Daniel Brewer wrote:
>> Hello,
>>
>> Does anyone have either the sequence information or an annotation table
>> for the Agilent Human 1 array (4100a)? I am trying to find accurate
>> location information for the probes but the internet seems to draw a
>> blank. I know that there is a 4100a annotation package but the location
>> info from this is via the gene and so may not be accurate
>> (probe->gene->location).
>
> Sorry, Daniel. What is the Agilent human 1 array? Is it the 44k oligo-based
> CGH array? I thought you had mentioned in another email that this was a cDNA
> array. Could you just specify how many probes are on the array and whether
> they are oligos or cDNAs?
>
> Sean
Don't be sorry. I am getting very confused myself. I believe it is
not the 44k oligo-based array and the it is a cDNA array.
The description from the paper where the data comes from:
"Commercial cDNA microarrays (Human 1.0;Agilent Technologies, Palo Alto,
CA) with 12,814 unique clones"
>From the data result files and the corresponding probe IDs I managed to
find the corresponding array in GEO (GPL876,
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL876) which
described it as "Agilent Human 1 cDNA Microarray (G4100A) [layout B]".
I have just noticed this in the description which might shead some light:
"Sourced from Incyte's UniGene 1 and Human Drug Target DNA clone sets".
So maybe the probe ID are Incyte clone IDs. Might be that is a path I
can follow but I've heard that getting Incyte clone information can be
tricky.
Sorry for the confusion. Hope that clears things up. Thanks for the help.
--
**************************************************************
Daniel Brewer, Ph.D.
Email: daniel.brewer at icr.ac.uk
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