[BioC] difference between illuminaHumanv1 and lumiHumanV1
Wolfgang Huber
huber at ebi.ac.uk
Tue Dec 18 16:23:45 CET 2007
Hi Burak
> Is there a difference between illuminaHumanv1 and lumiHumanV1 packages in terms of content?
> Thanks a lot
> -burak
Here is the situation afaiCs for illuminaHumanv2 and lumiHumanV2 packages:
- in lumiHumanV2, annotation environments are indexed by Pan Du's nuID
[1], which correspond 1:1 to reporter sequence IDs, and a translation
table is provided to Illumina *Probe* IDs. AfaIu, the package authors do
their own mapping of reporter sequences to the transcriptome.
- in illuminaHumanv2, annotation environments are indexed by the
Illumina *target* ID, which correspond to the intended (by Illumina)
target gene. See Lynn's message. With the current package version
1.4.0., the function illuminaHumanv2() returns strange "quality control"
results about "Probe number missmatch".
In both cases, I have not found documentation of the quality and
uniqueness of the matches of the reporters to the transcriptome.
[1] Du, P., Kibbe, W.A. and Link, S.M., "nuID: A universal naming
schema of oligonucleotides for Illumina, Affymetrix, and other
microarrays", submitted.
Best wishes
Wolfgang
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Wolfgang Huber EBI/EMBL Cambridge UK http://www.ebi.ac.uk/huber
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