[BioC] Affy genechips: probes many times
Saroj Mohapatra
smohapat at vbi.vt.edu
Tue Oct 3 16:57:22 CEST 2006
Hi friends:
I find that there are multiple instances of probes (i.e., the same
25-mer sequence) on the same array.
For example:
--------------
> library("hgu133atagprobe")
> seqnc <- hgu133atagprobe$sequence
-------------
There are 248152 probes, out of which 6067 are duplicated (the number
varies between 2 and 20).
-------------
> length(seqnc)
[1] 248152
> sum(duplicated(seqnc))
[1] 6067
> numTimes = sapply(unique(seqnc[duplicated(seqnc)]), function(x)
sum(seqnc==x))
> range(numTimes)
[1] 2 20
-------------
I have the question in mind: what is the logic behind multiple
instances of the same probes (e.g., quality control?)
I thank in advance for any feedback or pointer to literature.
Saroj
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