[BioC] Unable to get expression-values from cel-files of yeast
Roman Brunnemann
brunnemann at mpimp-golm.mpg.de
Fri Mar 3 16:14:35 CET 2006
Hi.
I'm working with bioconductor since 2 month and I never had any
problems. I used CEL-Files from ATGenExpress and everything was okay.
Now I tried to analyse different CEL-Files of yeast. I used different
sources (
http://chemogenomics.stanford.edu/supplements/01yfh/files/raw_data/tar_archives/
http://www.brownlab.info/publications/Brown2005/celfiles/index.htm
)
But I didn't succeeded to make an
library(affy) and than data <- justRMA() in the cel-file directory.
On two different machines I got two different errors. The first computer
says:
##########################
> data <- justRMA()
Error in getCdfInfo(object) : Could not obtain CDF environment, problems
encountered:
Specified environment does not contain DNA_TAGS_3
Library - package dnatags3cdf not installed
Data for package affy did not contain dnatags3cdf
Bioconductor - dnatags3cdf not available
##########################
and the second one says:
##########################
library(affy)
Loading required package: Biobase
Loading required package: tools
Welcome to Bioconductor
Vignettes contain introductory material. To view,
simply type: openVignette()
For details on reading vignettes, see
the openVignette help page.
Loading required package: reposTools
> data <- justRMA()
Note: BIOCRel1.8/src/contrib does not seem to have a valid repository,
skipping
Warning:
Failed to read replisting at BIOCRel1.8/src/contrib in:
getReplisting(repURL, repFile, method = method)
Note: BIOCRel1.8/bin/windows/contrib/2.3 does not seem to have a valid
repository, skipping
Warning:
1: Failed to read replisting at BIOCRel1.8/bin/windows/contrib/2.3 in:
getReplisting(repURL, repFile, method = method)
2: Failed opening: HTTP Status war '404 Not Found'
Note:
http://www.bioconductor.org/packages/data/annotation/stable/bin/windows/contrib/2.3 does not seem to have a valid repository, skipping
Note: Using download type: Source
Fehler in getCdfInfo(object) : Could not obtain CDF environment,
problems encountered:
Specified environment specified did not contain DNA_TAGS_3
Library - package dnatags3cdf not installed
Data for package affy did not contain DNA_TAGS_3
Bioconductor - dnatags3cdf not available
Warning:
Failed to read replisting at
http://www.bioconductor.org/packages/data/annotation/stable/bin/windows/contrib/2.3 in: getReplisting(repURL, repFile, method = method)
##############################
Maybe anyone had the same problem allready and can help me out of this.
Thanks in advance.
Best regards. Roman Brunnemann
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