[BioC] a problem with package limma (read.maimages)

[Gordon Smyth]

>Date: Fri, 16 Jun 2006 14:20:37 +0200
>From: Svetlana Bulashevska <s.bulashevska at dkfz-heidelberg.de>
>Subject: [BioC] a problem with package limma (read.maimages)
>To: bioconductor at stat.math.ethz.ch
>Message-ID: <4492A215.5070105 at dkfz.de>
>Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
>Dear Sirs,
>I would greatly appreciate if you could help me with my problem.
>I try to read in Genepix data and have a problem with the function
>read.maimages,

What problem exactly?

>even if I list the columns explicitely like this:
>RG <-read.maimages(files,source="genepix",columns=list(Gf="F532
>Mean",Gb="B532 Median"),annotation=c("Block","Column","Row","Name","ID"))
>I guess I have different column heading in my image output file as is
>required in the
>function and some columns are absent.

You guess or you know? Are you saying you have single-channel GenePix data?

Best wishes
Gordon

>  Could you please tell me how I can
>avoid this problem.
>Thank you,
>Svetlana Bulashevska.