[BioC] Farms library
phguardiol at aol.com
phguardiol at aol.com
Fri Jun 16 21:09:34 CEST 2006
Hi,
I ve been trying the new FARMS library to analyse primary signals of
Affy U133A chips (using R2.4dev downloaded yesterday on a PC with
winXPPro) which according to a recent publication in Bioinformatics is
more accurate than RMA / gcRMA etc...
Why is it that I get for more than 3/4 of the probesets the same signal
values for all chips for a given probesets ? and this in multiple
different set of chips. Such as probeset1 gave for chip1, 2, 3, etc...
the same intensity values for all chips ??? Is it because of the
normalisation process proposed here ? I m septic...
BTW, I used the q.farms function there in such a way:
data1 <- ReadAffy()
data2 <- q.farms(data1)
thank for the help
regards
Philippe Guardiola, MD
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