[BioC] median of the NUSE value
Ben Bolstad
bmb at bmbolstad.com
Thu Jun 15 18:42:16 CEST 2006
Below is my results using affyPLM 1.9.5. No particular problem here
with that version. What was your fitPLM call?
> Pset <- fitPLM(Dilution)
Background correcting PM
Normalizing PM
Fitting models
> NUSE(Pset,main="NUSE values",col=rainbow(4))
> NUSE(Pset,type="stats")
20A 20B 10A 10B
median 0.99929158 0.99939860 1.00213287 0.99927522
IQR 0.02592881 0.02963567 0.02816327 0.02591606
> NUSE(Pset,type="stats")
20A 20B 10A 10B
median 0.99929158 0.99939860 1.00213287 0.99927522
IQR 0.02592881 0.02963567 0.02816327 0.02591606
On Thu, 2006-06-15 at 13:11 +0200, kfbargad at ehu.es wrote:
> Dear list,
>
> I am using affyPLM for my QC, and am trying to obtain the median of
> the NUSE values for my chips.
>
> if I type
> >NUSE(Pset, main = "NUSE values")
> I get the NUSE boxplot
>
> but if I type
> > NUSE(Pset, type = "stats")
> Error in match.arg(type) : 'arg' should be one of plot, values
> or
> > NUSE(Pset, type = "values")
> Error in x at weights[which, ] : incorrect number of dimensions
> In addition: Warning messages:
> 1: longer object length
> is not a multiple of shorter object length in: model == (PM ~ -
> 1 + probes + samples)
> 2: longer object length
> is not a multiple of shorter object length in: model == (PM ~ -
> 1 + samples + probes)
> 3: the condition has length > 1 and only the first element will be
> used in: if ((model == (PM ~ -1 + probes + samples)) | (model == (PM ~
>
> my sessionInfo()
> Version 2.3.0 (2006-04-24)
> i386-pc-mingw32
>
> attached base packages:
> [1] "stats" "graphics" "grDevices" "utils" "datasets" "tools"
> "methods" "base"
>
> other attached packages:
> hgu133plus2cdf affyPLM gcrma matchprobes
> affydata affy affyio Biobase
> "1.12.0" "1.8.0"
>
>
> Any suggestions??
>
> Also, is it possible to colour the boxes of the boxplot? I have so far
> been using
> boxplot() for Pset with the argument "col", but it does not work for
> NUSE()
>
>
> Thanks in advance,
>
> David
>
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--
Ben Bolstad <bmb at bmbolstad.com>
http://bmbolstad.com
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