[BioC] limma: duplicateCorrelation
Gordon K Smyth
smyth at wehi.EDU.AU
Thu Jun 15 02:01:39 CEST 2006
> Date: Tue, 13 Jun 2006 10:22:08 +0200
> From: Christelle Dantec <Christelle.Dantec at igf.cnrs.fr>
> Subject: [BioC] limma: duplicateCorrelation
> To: bioconductor at stat.math.ethz.ch
> Content-Type: text/plain
>
> Hi,
>
> I have a technical replicates with dye-swap as:
> Cy3 Cy5
> -------------
> wt1 mu1
> mu1 wt1
> wt2 mu2
> mu2 wt2
> wt3 mu3
> mu3 wt3
> wt4 mu4
> mu4 wt4
>
>
>
> designM <- c(1,-1,1,-1,1,-1,1,-1)
> corfit<- duplicateCorrelation(MAlist, designM, bloc=c(1,1,2,2,3,3,4,4))
> fit<- lmFit(MAlist,design=designM,block=c(1,1,2,2,3,3,4,4),
> correlation=corfit$consensus)
>
> but I have this error message:
> Running plug-in: normalization.R
> Warning in Too much damping - convergence tolerance not achievable
> Error in paste(errMsg, "\n", sep = "") : argument "errMsg" is missing,
> with no default
> Execution halted
> Failed to execute R-script
>
> This error appears when creating the corfit variable.
> If someone can help me...
>
> Thanks
>
> Christelle
Dear Christelle,
It would appear from the error messsage that you are not using limma directly but rather are using
a third-party "plug-in". The error message is occuring in the plug-in, not in the underlying
limma functions. You should raise the problem with the author of the plug-in.
Best wishes
Gordon
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