[BioC] AnnBuilder doesn't work on gene information ("GENENAME", "SYMBOL", etc)
Luo Weijun
luo_weijun at yahoo.com
Thu Jul 13 20:31:35 CEST 2006
Thank you, John. Shall I use 2-column or 3-column base
file for the new version of AnnBuilder? And the new
version number will be 1.10.2 or 1.10.3? I have
already seen an OS X binary version shown up in
AnnBuilder website,
http://www.bioconductor.org/packages/bioc/1.8/html/AnnBuilder.html,
I am not sure whether that's the new version.
Weijun
--- John Zhang <jzhang at jimmy.harvard.edu> wrote:
>
> >I installed the updated version of AnnBuilder
> >successfully following your suggestion. The bad
> news
> >is that I still don¡¯t have the gene information,
> >xxACCNUM, xxGENENAME, xxSYMBOL,
> >xxGO, xxOMIM, xxPMID, etc, in my built annotation
> >package, after I inserted one empty column between
> >probe names and Entrez Gene ID columns in my base
> >file. Here is what I got:
>
> I have fixed the code. You may want to wait to use
> the new version off the
> developmental track.
>
>
>
> >
> >> library(hs95av2Entrezg7)
> >> hs95av2Entrezg7()
> >
> >
> >Quality control information for hs95av2Entrezg7
> >Date built: Created: Thu Jul 13 12:25:38 2006
> >
> >Number of probes: 8359
> >Probe number missmatch: None
> >Probe missmatch: None
> >Mappings found for probe based rda files:
> > hs95av2Entrezg7ACCNUM found 0 of 8359
> > hs95av2Entrezg7CHRLOC found 8042 of 8359
> > hs95av2Entrezg7ENZYME found 1315 of 8359
> > hs95av2Entrezg7LOCUSID found 8292 of 8359
> > hs95av2Entrezg7PATH found 2785 of 8359
> >Mappings found for non-probe based rda files:
> > hs95av2Entrezg7CHRLENGTHS found 25
> > hs95av2Entrezg7ENZYME2PROBE found 652
> > hs95av2Entrezg7ORGANISM found 1
> > hs95av2Entrezg7PATH2PROBE found 180
> > hs95av2Entrezg7PFAM found 6934
> > hs95av2Entrezg7PROSITE found 4941
> >
>
>>a=read.delim('/Users/luow/project/microarraydata/annotation/hs95av2Entrezg7Bas
> e.txt',sep='\t',
> >head=F)
> >> head(a)
> > V1 V2 V3
> >1 10001_at NA 10001
> >2 10002_at NA 10002
> >3 10003_at NA 10003
> >4 10004_at NA 10004
> >5 10005_at NA 10005
> >6 10006_at NA 10006
> >>
> >Below is my sessionInfo when I build the
> annotation.
> >Actually before I updated AnnBuilder, the same
> thing
> >happened when I used a 3-column base file. It seems
> to
> >me that to get those gene information data, our
> >current llParser needs GenBank accession numbers in
> >the 2nd column. But it doesn¡¯t make too much sense
> to
> >me, since to pull out gene information from NCBI
> >Entrez Gene database, we just need any one of the
> >commonly used gene IDs¡
> >Please let me know what I can do about this. Thank
> you
> >again for all your kind help.
> >Weijun
> >
> >> sessionInfo()
> >Version 2.3.1 (2006-06-01)
> >powerpc-apple-darwin8.6.0
> >
> >attached base packages:
> >[1] "tools" "methods" "stats" "graphics"
> >"grDevices" "utils"
> >[7] "datasets" "base"
> >
> >other attached packages:
> >AnnBuilder RSQLite DBI annotate
> XML
> > Biobase
> > "1.10.2" "0.4-1" "0.1-10" "1.10.0"
> "0.99-7"
> > "1.10.0"
> >>
> >
> >
> >--- nli at fhcrc.org wrote:
> >
> >> Sorry, the OS X version of AnnBuilder v1.10.2 is
> not
> >> available yet. We will try
> >> to get it ready as soon as possible.
> Alternatively,
> >> you can download the source
> >> from
> >>
>
>http://www.bioconductor.org/packages/bioc/1.8/html/AnnBuilder.html
> >> and
> >> install it yourself. The modification that
> matters
> >> to you is in
> >> AnnBuilder/R/GP.R .
> >>
> >> best
> >>
> >> nianhua
> >>
> >> Quoting Luo Weijun <luo_weijun at yahoo.com>:
> >>
> >> > Thank you so much, Nianhua,
> >> > I think it works now. One more question, You
> >> suggested
> >> > me to update AnnBuilder to the lastest version,
> >> > 1.10.1, since The UCSC Genome
> >> > database has updated. But I am not sure exactly
> >> how to
> >> > do it. I tried to update AnnBuilder package
> with
> >> > biocLite('AnnBuilder'), as well as using
> >> > update.packages(repos=biocReposList())
> following
> >> > Seth¡¯s suggestion, all I got is still the old
> >> > version, AnnBuilder_1.10.0. Therefore, could
> you
> >> tell
> >> > me how can I update to the lastest AnnBuilder
> >> version?
> >> >
> >> > This is my session info after
> >> > update.packages(repos=biocReposList())
> >> >
> >> > > sessionInfo()
> >> > Version 2.3.1 (2006-06-01)
> >> > powerpc-apple-darwin8.6.0
> >> >
> >> > attached base packages:
> >> > [1] "tools" "methods" "stats"
> "graphics"
> >>
> >> > "grDevices" "utils"
> >> > [7] "datasets" "base"
> >> >
> >> > other attached packages:
> >> > AnnBuilder RSQLite DBI annotate
>
> >> XML
> >> > Biobase
> >> > "1.10.0" "0.4-1" "0.1-10" "1.10.0"
> >> "0.99-7"
> >> > "1.10.0"
> >> > >
> >> > I really appreciate your help!
> >> > Weijun
> >> >
> >> >
> >> >
> __________________________________________________
> >> > Do You Yahoo!?
> >
> >> protection around
> >> > http://mail.yahoo.com
> >> >
> >>
> >>
> >>
> >>
> >>
> >
> >_______________________________________________
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>
> Jianhua Zhang
> Department of Medical Oncology
> Dana-Farber Cancer Institute
> 44 Binney Street
> Boston, MA 02115-6084
>
>
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