[BioC] AnnBuilder doesn't work on gene information ("GENENAME", "SYMBOL", etc)
Luo Weijun
luo_weijun at yahoo.com
Thu Jul 13 19:05:16 CEST 2006
Hello Nianhua,
I installed the updated version of AnnBuilder
successfully following your suggestion. The bad news
is that I still don¡¯t have the gene information,
xxACCNUM, xxGENENAME, xxSYMBOL,
xxGO, xxOMIM, xxPMID, etc, in my built annotation
package, after I inserted one empty column between
probe names and Entrez Gene ID columns in my base
file. Here is what I got:
> library(hs95av2Entrezg7)
> hs95av2Entrezg7()
Quality control information for hs95av2Entrezg7
Date built: Created: Thu Jul 13 12:25:38 2006
Number of probes: 8359
Probe number missmatch: None
Probe missmatch: None
Mappings found for probe based rda files:
hs95av2Entrezg7ACCNUM found 0 of 8359
hs95av2Entrezg7CHRLOC found 8042 of 8359
hs95av2Entrezg7ENZYME found 1315 of 8359
hs95av2Entrezg7LOCUSID found 8292 of 8359
hs95av2Entrezg7PATH found 2785 of 8359
Mappings found for non-probe based rda files:
hs95av2Entrezg7CHRLENGTHS found 25
hs95av2Entrezg7ENZYME2PROBE found 652
hs95av2Entrezg7ORGANISM found 1
hs95av2Entrezg7PATH2PROBE found 180
hs95av2Entrezg7PFAM found 6934
hs95av2Entrezg7PROSITE found 4941
>a=read.delim('/Users/luow/project/microarraydata/annotation/hs95av2Entrezg7Base.txt',sep='\t',
head=F)
> head(a)
V1 V2 V3
1 10001_at NA 10001
2 10002_at NA 10002
3 10003_at NA 10003
4 10004_at NA 10004
5 10005_at NA 10005
6 10006_at NA 10006
>
Below is my sessionInfo when I build the annotation.
Actually before I updated AnnBuilder, the same thing
happened when I used a 3-column base file. It seems to
me that to get those gene information data, our
current llParser needs GenBank accession numbers in
the 2nd column. But it doesn¡¯t make too much sense to
me, since to pull out gene information from NCBI
Entrez Gene database, we just need any one of the
commonly used gene IDs¡
Please let me know what I can do about this. Thank you
again for all your kind help.
Weijun
> sessionInfo()
Version 2.3.1 (2006-06-01)
powerpc-apple-darwin8.6.0
attached base packages:
[1] "tools" "methods" "stats" "graphics"
"grDevices" "utils"
[7] "datasets" "base"
other attached packages:
AnnBuilder RSQLite DBI annotate XML
Biobase
"1.10.2" "0.4-1" "0.1-10" "1.10.0" "0.99-7"
"1.10.0"
>
--- nli at fhcrc.org wrote:
> Sorry, the OS X version of AnnBuilder v1.10.2 is not
> available yet. We will try
> to get it ready as soon as possible. Alternatively,
> you can download the source
> from
>
http://www.bioconductor.org/packages/bioc/1.8/html/AnnBuilder.html
> and
> install it yourself. The modification that matters
> to you is in
> AnnBuilder/R/GP.R .
>
> best
>
> nianhua
>
> Quoting Luo Weijun <luo_weijun at yahoo.com>:
>
> > Thank you so much, Nianhua,
> > I think it works now. One more question, You
> suggested
> > me to update AnnBuilder to the lastest version,
> > 1.10.1, since The UCSC Genome
> > database has updated. But I am not sure exactly
> how to
> > do it. I tried to update AnnBuilder package with
> > biocLite('AnnBuilder'), as well as using
> > update.packages(repos=biocReposList()) following
> > Seth¡¯s suggestion, all I got is still the old
> > version, AnnBuilder_1.10.0. Therefore, could you
> tell
> > me how can I update to the lastest AnnBuilder
> version?
> >
> > This is my session info after
> > update.packages(repos=biocReposList())
> >
> > > sessionInfo()
> > Version 2.3.1 (2006-06-01)
> > powerpc-apple-darwin8.6.0
> >
> > attached base packages:
> > [1] "tools" "methods" "stats" "graphics"
>
> > "grDevices" "utils"
> > [7] "datasets" "base"
> >
> > other attached packages:
> > AnnBuilder RSQLite DBI annotate
> XML
> > Biobase
> > "1.10.0" "0.4-1" "0.1-10" "1.10.0"
> "0.99-7"
> > "1.10.0"
> > >
> > I really appreciate your help!
> > Weijun
> >
> >
> > __________________________________________________
> > Do You Yahoo!?
> protection around
> > http://mail.yahoo.com
> >
>
>
>
>
>
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