[BioC] Predicting number of replicates

[Khan, Sohail]

Dear List,

I have a basic statistical question regarding a Microarray data set.  However, I am not an statistician.  I was told the following problem can be modeled in R/ S_PLUS.

I have a data set with 3 replicates for the control and 3 replicates for experimental samples for a particular experiment.  I performed a t.test and adjusted the p. values for the false discovery rate.  Choosing a p value < 0.05, I didn't see any significant difference between the control and the experimental samples.  My question is how can I take the existing data and sort of 'simulate" (add data points) so that the adjusted p values become significant (< 0.05). The data is follows:

C.1	C.2	C.3	E.1	E.2	E.3
9.33	8.97	9.03	10.12	9.84	9.65
7.35	6.98	7.14	8.36	9.30	9.02
......	.......	........	........	.......	.....	
.......	.......	........	........	.......	......

I thank you for any suggestions/advice...............any insight into this  would be a great learning experience for me.


Sohail Khan
Scientific Programmer
COLD SPRING HARBOR LABORATORY
Genome Research Center
500 Sunnyside Boulevard
Woodbury, NY 11797
(516)422-4076