[BioC] Help on Loading AgilentData into LIMMA
Gordon Smyth
smyth at wehi.edu.au
Thu Nov 3 02:46:17 CET 2005
You need to state the versions of R and limma that you are using. See
"While sending mail to the list": Item 4 of the posting guide at
http://www.bioconductor.org/docs/postingGuide.html
for a suggestion of how to do this.
Gordon
>[BioC] Help on Loading AgilentData into LIMMA
>Nataliya Yeremenko eremenko at science.uva.nl
>Tue Nov 1 23:43:30 CET 2005
>
>I need help on loading data to LIMMA
>I'm working with 44K Agilent microarrays.
>
>I'm novice in BioConductor, so I'm trying to follow instructions on the
>packages
>to deal with my data.
>
>I'm trying to load Feature Extraction raw data with
> > RG <- read.maimages(files = targets$fileName, path = loadPath, source
>= "agilent")
>and data seems to be loaded fine, where targets is experiment
>description file.
>It is loading all my 20 replicates,
>but when I checked RG data set with dim(RG) or show(RG)
>I realized that only 6195 rows have been loaded.
>It is the same situation independent on how many arrays I'm loading 1 or 28.
>Is there limit for number of spots in the package?
>
>
>I've tried to start as well with arrayMagic package
>and again didn't succeed to load even single array,
>After 10 min of loading I see error message stating
>that line 17000 has not proper amount of arguments.
>
>Thank you in advance for any suggestion
>
>--
>Dr. Nataliya Yeremenko
>
>Universiteit van Amsterdam
>Faculty of Science
>IBED/AMB (Aquatische Microbiologie)
>Nieuwe Achtergracht 127
>NL-1018WS Amsterdam
>the Netherlands
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