[BioC] LIMMA: uneven replication of samples

[Jakob Hedegaard]

Hi!

 

I am going to analyze data from an cDNA microarray experiment conducted
to compare "diseased" and "healthy" pigs.

The experiment has been conducted as a "classical common reference"
experiment with the reference labeled with the same dye on all slides. A
total of 24 animals are included in the experiment (12 "diseased" and 12
"healthy").

 

Question: one of the "diseased" and one of the "healthy" animals have
been replicated three times resulting in a total of 28 slides.

 

 

How should this replication be handled in the analysis using LIMMA?

 

 

Looking forward hearing from you!

Jakob

 

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Jakob Hedegaard

Danish Institute of Agricultural Sciences

Department of Genetics and Biotechnology

Molecular Genetics and System Biology

Building K25

Research Centre Foulum

P.O. box 50

DK-8830 Tjele

Denmark

Tel: (+45)89991363

Fax: (+45)89991300

 


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