[BioC] limma design matrix
Gordon Smyth
smyth at wehi.edu.au
Tue Jan 11 07:46:40 CET 2005
See limma User's Guide http://bioinf.wehi.edu.au/limma/usersguide.pdf
middle of page 32.
Gordon
Mon Jan 10 17:42:20 CET 2005
>Hi
>
>I know this has been dealt with before, but I would really appreciate some
>help.
>I am anaylsing affy chips and I have three different sample types,
>lpl-high, lpl-low and brain.
>I construct a factor vector below
>TS <-c(rep("lpl-high",12),rep("lpl-low",10),rep("brain",17))
>TS<- factor(TS, levels= c("lpl-high","lpl-low","brain"))
>
>I would simply want to make an analysis of variance, see wheter these
>three samples are differentially expressed (F value). I am not interested
>in any contrasts. How can I do that?
>
>Thanks a lot!!!!!
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