[BioC] something weird with read.maimages and .gpr files

[Pita]

what can trigger the following error? I have imported many genepix 
files  without any problem

 >> RG.new.gpr <- read.maimages(files.new,"genepix",wt.fun=wtflags(0.1))
 >Error in "[.data.frame"(obj, , columns$Gf) :
  >       undefined columns selected

This is with limma version 1.8.22


Peter

P.S. Here is a snippet of the first .gpr file:

ATF	1.0
29	56
"Type=GenePix Results 3"
"DateTime=2005/01/26 14:13:02"
"Settings="
"GalFile=C:\Axon\Params\ImmunArray2.gal"
"PixelSize=10"
"Wavelengths=635	594"
"ImageFiles=Y:\one\two\IMMUNOARRAY\26.01.05\13012006_SEKA0137-01_Cy5_26.01.05.tif.tif 
0	Y:\one\two\IMMUNOARRAY\26.01.05\13012006_HURR_Cy3_26.01.05.tif 0"
"NormalizationMethod=None"
"NormalizationFactors=1	1"
"JpegImage=C:\Axon\Data\13012006_SEKA0137-01_Cy5_0026.jpg"
"StdDev=Type 1"
"RatioFormulations=W1/W2 (635/594)"
"FeatureType=Circular"
"Barcode="
"BackgroundSubtraction=LocalFeature"
"ImageOrigin=0, 0"
"JpegOrigin=1190, 940"
"Creator=GenePix Pro 6.0.0.76"
"Scanner=4000 680056"
"FocusPosition=0"
"Temperature=0"
"LinesAveraged=1"
"Comment="
"PMTGain=0	0"
"ScanPower=100	100"
"LaserPower=0	0"
"Filters=	"
"ScanRegion=0,0,2022,3665"
"Supplier="
"Block"	"Column"	"Row"	"Name"	"ID"	"X"	"Y"	"Dia."	"F635 Median"	"F635 Mean" 
"F635 SD"	"F635 CV"	"B635"	"B635 Median"	"B635 Mean"	"B635 SD"	"B635 CV" 
"% > B635+1SD"	"% > B635+2SD"	"F635 % Sat."	"F594 Median"	"F594 Mean"	"F594 
SD"	"F594 CV"	"B594"	"B594 Median"	"B594 Mean"	"B594 SD"	"B594 CV"	"% > 
B594+1SD"	"% > B594+2SD"	"F594 % Sat."	"Ratio of Medians (635/594)"	"Ratio 
of Means (635/594)"	"Median of Ratios (635/594)"	"Mean of Ratios (635/594)" 
"Ratios SD (635/594)"	"Rgn Ratio (635/594)"	"Rgn R2 (635/594)"	"F Pixels" 
"B Pixels"	"Circularity"	"Sum of Medians (635/594)"	"Sum of Means 
(635/594)"	"Log Ratio (635/594)"	"F635 Median - B635"	"F594 Median - B594" 
"F635 Mean - B635"	"F594 Mean - B594"	"F635 Total Intensity"	"F594 Total 
Intensity"	"SNR 635"	"SNR 594"	"Flags"	"Normalize"	"Autoflag"
1	1	1	"DUSP2:Hs.1183:AA759046"	"1321598"	1540	1160	100	2528	2522	1254	49	0 
0	90	154	171	100	97	0	1971	1990	1077	54	0	0	59	123	208	100	97	0	1.283	1.267 
1.363	1.339	2.275	1.272	0.575	80	546	100	4499	4512	0.359	2528	1971	2522 
1990	201729	159237	15.792	15.699	0	0	0
1	2	1	"DUSP2:Hs.1183:AA759046"	"1321598"	1750	1170	120	2686	2625	1525	58	0 
0	84	174	207	97	95	0	1841	2014	1266	62	0	0	47	106	225	96	95	0	1.459	1.303 
1.352	1.417	2.660	1.326	0.630	120	693	100	4527	4639	0.545	2686	1841	2625 
2014	314976	241629	14.603	18.557	0	0	0
1	3	1	"MYL4:Hs.356717:AA705225"	"461425"	1940	1160	110	7604	7657	2219	28	0 
0	77	142	184	100	100	0	4055	4284	1390	32	0	0	65	133	204	100	100	0	1.875 
1.787	1.684	1.811	1.524	2.029	0.741	80	572	100	11659	11941	0.907	7604	4055 
7657	4284	612532	342716	53.380	31.722	0	0	0
1	4	1	"MYL4:Hs.356717:AA705225"	"461425"	2140	1160	110	6585	7117	2955	41	0 
0	84	167	198	100	100	0	3261	3319	1518	45	0	0	73	164	224	96	95	0	2.019	2.144 
2.060	2.423	2.458	2.355	0.736	80	620	100	9846	10436	1.014	6585	3261	7117 
3319	569339	265548	42.114	19.793	0	0	0
1	5	1	"PSMB8:Hs.180062:AA181300"	"624360"	2350	1160	110	2433	2535	1160	45	0 
0	79	146	184	100	98	0	2484	2641	1292	48	0	0	83	219	263	97	97	0	0.979	0.960 
0.951	0.989	2.062	0.908	0.637	80	579	100	4917	5176	-0.030	2433	2484	2535 
2641	202824	211288	16.822	11.680	0	0	0
1	6	1	"PSMB8:Hs.180062:AA181300"	"624360"	2540	1170	120	2155	2327	1336	57	0 
0	72	139	193	97	95	0	2260	2256	1207	53	0	0	53	129	243	95	92	0	0.954	1.031 
0.961	0.998	2.537	1.101	0.660	120	692	100	4415	4583	-0.069	2155	2260	2327 
2256	279193	270688	16.223	17.078	0	0	0
1	7	1	"RAD51C:Hs.412587:R37145"	"26997"	2750	1160	90	1743	1788	796	44	0	0 
76	143	188	100	98	0	2250	2460	1181	48	0	0	66	161	243	100	98	0	0.775	0.727 
0.719	0.730	2.097	0.617	0.472	52	420	100	3993	4248	-0.368	1743	2250	1788 
2460	92995	127944	11.972	14.870	0	0	0
1	8	1	"RAD51C:Hs.412587:R37145"	"26997"	2950	1160	100	1184	1237	725	58	0	0 
74	134	181	100	95	0	1938	1806	998	55	0	0	66	147	222	95	92	0	0.611	0.685 
0.579	0.692	2.521	0.599	0.484	80	540	100	3122	3043	-0.711	1184	1938	1237 
1806	98992	144478	8.679	11.837	0	0	0
1	9	1	"SHC1:Hs.433795:R52961"	"40303"	3140	1160	90	2265	2434	1153	47	0	0	83 
160	192	100	100	0	2064	2021	1040	51	0	0	73	140	191	98	96	0	1.097	1.204 
1.232	1.289	2.207	1.187	0.553	52	414	100	4329	4455	0.134	2265	2064	2434 
2021	126582	105071	14.694	13.914	0	0	0
1	10	1	"SHC1:Hs.433795:R52961"	"40303"	3350	1170	100	2040	2032	1088	53	0	0 
75	122	162	100	100	0	1771	1847	1021	55	0	0	61	119	195	98	96	0	1.152	1.100 
1.104	1.141	2.092	1.121	0.569	80	532	100	3811	3879	0.204	2040	1771	2032 
1847	162549	147753	16.041	15.008	0	0	0
1	11	1	"TYROBP:Hs.9963:H12338"	"148469"	3550	1170	110	15761	14143	5861	41 
21	21	106	172	162	100	100	0	744	884	592	66	0	0	42	101	240	91	86	0	21.156 
15.975	17.541	19.010	2.619	18.419	0.594	80	590	100	16484	15006	4.403	15740 
744	14122	884	1131446	70752	81.610	8.337	0	0	0
1	12	1	"TYROBP:Hs.9963:H12338"	"148469"	3750	1170	110	15557	14415	6677	46	0 
0	103	178	172	100	100	0	865	916	602	65	0	0	47	131	278	90	80	0	17.985	15.737 
16.305	18.014	2.349	18.037	0.619	80	590	100	16422	15331	4.169	15557	865 
14415	916	1153181	73243	80.404	6.634	0	0	0