[BioC] masking probe pairs in RMA
Lana Schaffer
schaffer at scripps.edu
Thu Feb 10 22:39:05 CET 2005
Greetings,
I have masked 168 probe pairs and then run RMA and Alot of the expression values have come out NA.
I am concluding that RMA is not calculating the expression values for probesets which contain masked
probepairs. Is this a correct conclusion?
abatch <- bg.correct.rma(abatch0);
msk <- scan(filename, skip=0, list("", ""))
for (i in 1:length(msk[[1]])) {
nam <- msk[[1]][i];
txt <- gsub("-", ":", msk[[2]][i]);
lst <- eval(parse(text=paste("c(", txt, ")"))); #list
if (i == 1) {
ids <- pmindex(abatch, nam)[[1]][lst];
} else {
ids <- c(ids, pmindex(abatch, nam)[[1]][lst]);
}
ids <- c(ids, mmindex(abatch, nam)[[1]][lst]);
}
intensity(abatch)[ids, ] <- NA;
eset2 <- rma(abatch,background=F);
Thanks for any help.
Lana
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