bioinfovijayaraj at yahoo.com
Sat Apr 30 20:49:04 CEST 2005
we are designing a microarray experiment, where there are two different mouse strains (A,B)...
and one condition (Peptide - P and Saline - S). we expect the mouse strains to express differentially even under normal (saline) conditions...so we did not want to go for pooling the controls, to have a common - pooled control... under this scenario...
which of the following designs would you suggest....(we have planned to use dyeswap and 2 replicates for each hybrisation)....
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(all pairs - with 6 hybridisations)
(with just 3 hybridisations, so that we could deduce AP-BP,using AP-AS-BS and rest like that...)
i request your valuable advice in this regard....
department of biological sciences
University of Southern Mississippi
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