[BioC] Spot quantitation
Jason Skelton
jps at sanger.ac.uk
Wed Apr 27 14:12:47 CEST 2005
Hi
I was wondering if anyone has any opinions about the SPOT spotfinding
software from csiro
(I.e the package that runs in R) and it's limitations, benefits, opinion
about batch performance accuracy etc.
Has anyone done any direct comparisons of SPOT with Genepix or other
spotfinding software avalible ?
Or is anyone routinely using SPOT and highly recommend it...
I'm currently comparing it to genepix data from a relatively old version
of genepix with kooperberg background subtraction
and using limma for all subsequent analysis.
many thanks
Jason
--
--------------------------------
Jason Skelton
Pathogen Microarrays
Wellcome Trust Sanger Institute
Hinxton
Cambridge
CB10 1SA
Tel +44(0)1223 834244 Ext 7123
Fax +44(0)1223 494919
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