[BioC] how to do with global shift
ramasamy at cancer.org.uk
Mon Apr 11 11:13:52 CEST 2005
This depends on how you call your genes to be affected. e.g. fold
change, t-test and at what threshold. What preprocessing did you try ?
Alternatively you simply rank the genes and see if the top/bottom 10,
20, 100 etc makes sense to you.
AFAIK, Affymetrix preprocessing algorithms do not necessarily have to
give equal numbers of up and down regulated genes. In fact in one of the
projects that we are involved in, of the final list of 200-300 genes we
found 95% of the genes were down regulated. Some of these were
successfully verified with taqman and the biologists were happy.
I am not a biologist but from what I understand normalization based on
house keeping genes are generally not recommended because they may not
be house keeping genes in the tissue that you are testing for.
On Fri, 2005-04-08 at 13:10 -0400, Dapeng Cui wrote:
> Dear Bioconductor users,
> Firstly I'm sorry if this subject is appropriate for the list. I remember there
> were some discussion in this list on what to do when treatment produces a
> global shift on gene expression. Now I'm facing this problem on T cell
> activation experiment. Upon activation T cells change dramaticaly. They start
> proliferation, getting much bigger and make more RNA. After running affy chips,
> whatever normalizations I did, thousands genes(20%~30%) on chip changed. Of
> course they all give equal number of up- and down-regulated genes. I feel
> really hard to make explantation from these data. And I think it's dangerous to
> normalize using housekeeping genes because HKs also change after T cell
> activation. Could anybody please give me some clues/publications on which
> pre-process/normalization works better for this situation?
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