[BioC] loged data or not loged previous to use normalize.quantile
Marcelo Luiz de Laia
mlaia at fcav.unesp.br
Fri Apr 1 20:20:17 CEST 2005
Dear Bioconductors Friends,
I have a question that I dont found answer for it. Please, if you have a
paper/article that explain it, please, tell me.
I normalize our data using normalize.quantile function.
If I previous transform our intensities (single channel) in log2, I dont
get differentially genes in limma.
But, if I dont transform our data, I get some genes with p.value around
0.0001, thats is great!
Of course, when I transform the intensities data to log2, I get some NA.
Why are there this difference? Am I wrong in does an analysis with not
Thanks a lot
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