[BioC] Is it right?

Caimiao Wei caimiaow at u.washington.edu
Fri Sep 17 18:10:22 CEST 2004


it depends on whether the contol is of interest.  if the objective of this 
study is to compare the differences between control and treated samples, 
then the design described below is not appropriate.  However, it is OK if 
the researcher is not interested in the control sample at all.

Caimiao

----- Original Message ----- 
From: "michael watson (IAH-C)" <michael.watson at bbsrc.ac.uk>
To: "Kaushik, Narendra K" <n.kaushik at imperial.ac.uk>; 
<bioconductor at stat.math.ethz.ch>
Sent: Friday, September 17, 2004 1:07 AM
Subject: RE: [BioC] Is it right?


> This is (quite) common practice.  The pooled sample represents a common
> reference for the treated samples.  A common reference experimental
> design *can* have certain advantages over direct comparisons, such as
> easy extensibility and ease of interpretation.  Pooling the control
> samples simply ensures that the common reference is more generic
> (hopefully) and lights up more spots (hopefully).
>
> Mick
>
> -----Original Message-----
> From: Kaushik, Narendra K [mailto:n.kaushik at imperial.ac.uk]
> Sent: 16 September 2004 22:56
> To: bioconductor at stat.math.ethz.ch
> Subject: [BioC] Is it right?
>
>
> Hi Folks,
>
> I have read an article on two color microarray hybridization. The
> authors have pooled control samples (1), this mixture was lablled,
> hybridized together with separately labelled treated (x) samples on x
> microarrays ( Treated samples were not pooled). Do you think that is
> good idea?
>
> Narendra
> Imperial College of Medicine,
> London W2 1PG
> UK
>
>
>
> [[alternative HTML version deleted]]
>
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